Figure 4: E. coli cells lacking Lhr are sensitive to oxidative stress
(A ) Viability spot tests showing moderately increased sensitivity of Δlhr cells to AZT (7.5 µg/mL) compared with wild type (wt) cells, and (B ) represented in viability curves when Δlhr and wild type cells were grown independently in media containing AZT at 2.5, 5, 7.5 or 10 µg/mL. The plots show grow relative to wild type cells grown in media lacking AZT.
(B ) Growth of Δlhr and wild type cells monitored in 96 well plates in media containing 12.5 mM H2O2, and (C ) viability spot tests comparing Δlhr and wild type cells grown in H2O2 added to media at 1.5625, 3.75, 6.25 and 12.5 nM.