DNA Extraction
DNA was extracted from midguts, carcasses or legs. DNA extraction was
performed with ZymoBiomics 96 MagBead DNA Kit (Zymo Research) for
midguts and with HighPrep Blood & Tissue DNA Kit (MagBio) for carcasses
and legs. Samples were mixed using 0.5 mm glass beads in 2 mL screwcap
tubes with a Precellys Evolution (Bertin Technologies) bead beater
homogenizer. Automatic DNA extraction was performed with the KingFisher
Duo Prime system (Thermo Scientific). DNA was eluted in ZymoBiomics
DNAse/RNAse Free Water from the kit for midguts and MB Elution Buffer
supplied in the kit for carcasses and legs. Extracted DNA was kept at
-80 °C until further utilization.