Capillary Electrophoresis
Capillary electrophoresis was carried out in a QIAxcel Advanced System
(Qiagen). QIAxcel DNA High Resolution Kit was chosen for higher
precision and samples were run using OM800 method, QX Alignment Marker
15 bp – 1 kb and QX DNA Size Marker 50 – 800 bp. Results were treated
with QIAxcel ScreenGel Software and manually checked to ensure that
detected peaks were real and that noise signals were eliminated. Reports
were generated and ITS2 amplicon lengths were recovered for further
analysis.