Mosquito Sampling
Mosquitoes were sampled in five different locations in French Guiana: La Césarée (CE) [5° 0’ 57.32” N, 52° 31’ 41.36” W], Le Galion (GA) [4° 47’ 0.08” N, 52° 24’ 42.74” W], Cacao (CA) [4° 34’ 32.59” N, 52° 28’ 4.81” W], Blondin (BL) [3° 52’ 37.21” N, 51° 48’ 48” W] and Trois Palétuviers (TP) [4° 2’ 59.46” N, 51° 40’ 9.77” W](Figure S1) . While CE, GA and CA are considered as malaria-free areas, BL and TP are located in a transborder region with higher risks of malaria resurgence. In 2017, a malaria outbreak was observed among inhabitants of TP village [38] , thus it remains a region of interest considering malaria control programs.
Field missions took place over a four-year period, from July to November of 2018, 2019, 2020 and 2021. Mosquitoes were collected using Mosquito Magnet traps (WoodStreamTM). Mosquito tissues were preserved in 70 to 100 % ethanol and kept at -80 °C upon arrival at the laboratory until further processing.