3.5 Determination of multiple bioactive constituents
The main bioactive components in TH are reported to be phenolic acids
and flavonoids [27,28]. To investigate the potential relationship
between metabolite accumulation and protein expression, the content of
main active constituents was determined simultaneously by
UFLC-QTRAP-MS/MS. The condition of UFLC-QTRAP-MS/MS and method
validation studies were reported in a previous study [14]. A total
of 16 constituents including 12 flavonoids and 4 phenolic acids were
quantitated in TH from two SS and FXS. The contents of major flavonoid
glycosides (quercitrin, hyperoside, auicularin,
quercetin-3-O -β -D -glucuronide, and rutin), and
(+)-Catechin in TH from SS were higher than those in TH from FXS. There
was no significant difference between the two groups in the content of
other constituents (Figure 5 and Table S8).