Abstract
Taxilli Herba (TH) is famous for its semi-parasitic feature, with
hundreds of host species. The molecular mechanisms governing the
accumulation of bioactive constituents in TH from different hosts have
yet to be completely understood. The purpose of this study was to
investigate the differences in synthesis and accumulation of bioactive
constituents in TH from Morus alba L. and Liquidambar
formosana Hance. The proteome of TH from two hosts were compared, and
proteins were identified using tandem mass tag (TMT) technology. Sixteen
bioactive constituents were quantitatively determined and analyzed in
conjunction with the results of proteomics. A total of 5681 proteins
were detected, of which 259 were significantly up-regulated and 274
significantly down-regulated in expression levels. Differentially
expressed proteins (DEPs) were shown to be mostly engaged in cellular
process and metabolic process by GO analysis. DEPs were considerably
abundant in amino acid metabolic pathways, according to KEGG pathway
analysis. The changes in the contents of 16 bioactive constituents were
mostly compatible with the expression patterns of relevant structural
proteins. The results of this study will provide basic information for
the role of protein in the secondary metabolism biosynthesis pathway and
the molecular mechanism of the quality formation of TH.