3.6 Parallel reaction monitoring verification
To verify the abundance of candidate proteins, PRM was used to conformed
validate 17 proteins (COMT, HCT, PRDX6, FLS, REF1, F3H, ANR, C3’H, PAL,
CYP75B1, LAR, CHI, bglB, CCoAOMT, ANS, CYP73A, and E1.11.1.7), as
determined by TMT data. Only the PRM results of PAL, COMT, HCT, PRDX6,
REF1 and C3’H were accordance with the proteomic data (Figure 6).
However, most of the fold changes in protein abundance detected by PRM
were larger than those detected by TMT data.