2.6 Bioinformatic analysis
Protein sequence alignment was performed by comparison with the databases Uniprot (version 2021.09), NCBI_NR (version 2021.09) and Swissprot (version 2021.06) databases. Principal component analysis (PCA) was carried out to distinguish the samples from two hosts. Gene Ontology (GO, version 2021.0918) was used for functional annotation to learn the functions of proteins in biological process (BP), cellular component (CC) and molecular function (MF) by BLAST2GO (version 2.5.0). Kyoto Encyclopedia of Genes and Genomes (KEGG, version 2021.09) was used to explain the involvement of proteins in biological pathways by KOBAS (version 2.1.1). Goatools (version 0.6.5) and Python (version 3.11.2) were applied for enrichment analysis. Hierarchical clustering analysis of protein was performed by Cluster (version 3.0) based on Pearson’s correlations.