3.2 GO functional annotation classification and enrichment analysis of DEPs
The DEPs was annotated into BP, CC, and MF by GO annotation analysis. The top 20 GO terms were calculated on the basis of ascendingp -value ranking (Figure 2A and Table S3). The DEPs were mainly involved in BP, of which cellular process and metabolic process accounted for a higher proportion. Apart from this, biological regulation and response to stimulus account for the remaining portion. It indicated that the cells of TH from two hosts differed in physiological status somewhat. Furthermore, under the CC category, proteins were exclusively connected to cellular anatomical entity and protein-containing complex. Most DEPs participated in catalytic activity while others were related to binding and transporter activity during MF. It was evident that in TH from SS and FXS, the proteins with catalytic and binding functions undergo changes in major functions such as metabolism and cellular processes in cellular anatomical entity and protein-containing complex. In Figure 2B and Table S4, the top 20 GO enrichment histogram of the DEPs was presented. There were eight items enriched in BP, among which GO: 0006952 (defense response) and GO: 0042440 (pigment metabolic process), and GO: 0033013 (tetrapyrrole metabolic process) were enriched in more proteins (23, 13, and 11, respectively). There were 11 items that were enriched in MF, two of which had lower p -values than the others (GO: 0003824 (catalytic activity and GO: 0090729 (toxin activity) were lower. There was one entry enriched in CC, with GO: 0005887 (integral component of plasma membrane).