2.7 Parallel reaction monitoring analysis
Majorbio Bio-pharm Technology Co., Ltd. utilized PRM to validate the abundance of 17 candidate proteins related to the synthesis of secondary metabolites (Shanghai, China). For the PRM analysis, three biological replicates from each group were considered. Based on the TMT data, the signature peptides of the targeted proteins were identified, and only distinct peptide sequences were chosen. The chromatographic column, orbit trap and electrospray voltage, protein identification library, and raw data processing software used for the LC-MS analysis in this investigation were identical to those used for the TMT analysis.