3.4 No significant change on heart rate (HR) in DSS rats
There was no significant change in HR between each high-salt (8%) group
and the sham group. The heart rate was approximately 200 bpm in each
group. (Figure 5)
3.5 Water
consumption, natriuresis
and diuresis
All high-salt (8%) groups exhibited significantly higher water
consumption, urination, and urinary sodium excretion compared to the
sham low-salt group (P<0.001). (Figure 6-8)
Compared to the
vehicle group, all treatment groups
showed varying degrees of slight decrease in water consumption, with
statistical differences at certain time points (P<0.01,
P<0.05). (Figure 6).
The results of the natriuresis study demonstrated that the strongest
effect was observed on the first day of treatment, with significant
differences compared to the vehicle group (P<0.001,
P<0.01, P<0.05). As the treatment duration
progressed, the natriuretic effects of all treatment groups gradually
diminished. However, on the 28th day of treatment, the LCZ696 group and
the middle/high-dose groups of S086 still exhibited significant
natriuretic effects (P<0.01, P<0.05). (Figure 7).
Regarding diuresis, significant diuretic effects were observed on the
first day of treatment in the LCZ696 group, sacubitril group, and
middle/high-dose groups of S086, with statistically significant
differences (P<0.001, P<0.01). The EXP3174 group and
the low-dose group of S086 showed an increasing trend in diuresis but
without statistical significance. Over the course of treatment, the
diuretic effects of all treatment groups gradually weakened. However, on
the 28th day of treatment, the LCZ696 group and the middle/high-dose
groups of S086 still exhibited significant diuretic effects
(P<0.05). (Figure 8).