2.4 Dahl salt sensitive hypertensive rat model
Telemetry devices were implanted in rats to measure heart rate and 24-hour baseline blood pressure. The rats were divided into two groups, with one group receiving 0.3% salt forage as a sham and the other group receiving 8% salt forage to induce hypertension. After seven days, rats with an average 24-hour systolic blood pressure of ≥160 mmHg were chosen and randomly divided into seven groups based on their systolic blood pressure. The successful standard for generating a hypertensive model was an average 24-hour systolic blood pressure of ≥160 mmHg. The groups included a sham group (n=8), vehicle group (n=7), LCZ696 group (68mg/kg, n=7), EXP3174 group (35mg/kg, n=7), Sacubitril group (33mg/kg, n=7), S086 low dose group (8mg/kg, n=7), S086 middle dose group (23mg/kg, n=7), and S086 high dose group (68mg/kg, n=7). The LCZ696, EXP3174, Sacubitril, and S086 high dose groups received equimolar doses of compounds. The rats’ heart rate, blood pressure, natriuresis, and diuresis were monitored weekly, and dose administration occurred every day at 11:00-12:00 AM. (Figure 1)