<div>[Strength of agreement: κ index &lt;0, poor; 0-0.20, slight;
0.21-0.40, fair; 0.41-0.60, moderate; 0.61-0.80, substantial; 0.81-1.0,
almost perfect; SE: Standard Error; PA: Percent Agreement]</div><div>The assessment of rHyC based dot-ELISA at lower incubation temperature
(18 <sup>o</sup>C) and time (30 min) provided valid results
suitable for field diagnosis of GWFI with dilutions below 1:10. The
limited assessment of neat and lower dilutions of samples at lower
temperature and incubation period were found to exhibit faint dots at
neat and 1:2 dilution whereas clear distinction was observed at 1:10
dilution of known samples. Moreover, the positive control taken as a
weak positive sample based on microtitre ELISA (OD=0.537) provided valid
results at the above conditions.</div><div>Further for serosurveillance, the serum samples available in the ICAR-NF
laboratory were tested, which could clearly differentiate between
infested and uninfested animals. A total of 274 serum samples suspected
of GWFI were tested, out of which 83 samples were found to be positive
for GWFI.</div>