Reference Sera
Positive reference goat serum samples (n=109) were collected from farms and a municipal slaughterhouse from Union Territory of Jammu and Kashmir (North India), based on palpation of larvae in live animals and carcass examination. Negative goat sera (n=92) were procured from Punjab state of North India, where GWFI has never been reported. In order to rule out the cross reactivity of developed dot-ELISA, positive serum of several important parasitic and bacterial diseases of goats namely oestrosis, haemonchosis,fasciolosis, amphistomiosis, coenurosis, cryptosporidiosis, paratuberculosis, enterotoxaemia and brucellosis were assessed.