Reference Sera
Positive reference goat serum samples (n=109) were collected from farms
and a municipal slaughterhouse from Union Territory of Jammu and Kashmir
(North India), based on palpation of larvae in live animals and carcass
examination. Negative goat sera (n=92) were procured from Punjab state
of North India, where GWFI has never been reported. In order to rule out
the cross reactivity of developed dot-ELISA, positive serum of several
important parasitic and bacterial diseases of goats namely oestrosis,
haemonchosis,fasciolosis, amphistomiosis, coenurosis, cryptosporidiosis,
paratuberculosis, enterotoxaemia and brucellosis were assessed.