Conclusion
Dot-ELISA has several advantages over commercially available ELISA kits,
including simplicity of the procedure, no requirement of highly precise
equipment such as an ELISA reader, ability to interpret results
visually, low cost and high sensitivity and specificity. The test is
also desired to be amenable to sample pooling to cover herd level
screening through bulk samples which facilitates mass surveillance. The
results of the present study demonstrate that dot-ELISA may provide a
suitable alternative to plate ELISA for the serodiagnosis of GWFI at
regional centres. The present study reports the first dot-ELISA based on
rHyC of P. silenus which can support mass surveillance programs
at the field level to achieve sustainable control of GWFI.