Stability of developed Dot-ELISA strips
The stability of antigen dotted NCM strips was assessed at different time and temperature combinations with a panel of GWFI positive and negative sera. Strips were dotted with a fixed concentration of rHyC antigen (188 ng/dot), blocked with 5% SMP and stored in sealed 15 mL falcon tubes to avoid moisture at 4 °C and 37 °C, respectively for total six months. Stored strips were re-tested at every one month interval for their reactivity with reference serum samples.