[Strength of agreement: κ index <0, poor; 0-0.20, slight; 0.21-0.40, fair; 0.41-0.60, moderate; 0.61-0.80, substantial; 0.81-1.0, almost perfect; SE: Standard Error; PA: Percent Agreement]
The assessment of rHyC based dot-ELISA at lower incubation temperature (18 oC) and time (30 min) provided valid results suitable for field diagnosis of GWFI with dilutions below 1:10. The limited assessment of neat and lower dilutions of samples at lower temperature and incubation period were found to exhibit faint dots at neat and 1:2 dilution whereas clear distinction was observed at 1:10 dilution of known samples. Moreover, the positive control taken as a weak positive sample based on microtitre ELISA (OD=0.537) provided valid results at the above conditions.
Further for serosurveillance, the serum samples available in the ICAR-NF laboratory were tested, which could clearly differentiate between infested and uninfested animals. A total of 274 serum samples suspected of GWFI were tested, out of which 83 samples were found to be positive for GWFI.