Western blotting
Left atrial tissues were collected after isolated heart perfusion, and
were homogenized using a tissue lyser. Levels of RyR2 (ryanodine
receptor, LS-C93425, LifeSpan BioSciences, USA), SERCA2 (sarcoplasmic
reticulum calcium pump, 4388s, Cell Signaling Technology, USA) and NCX1
(Na+/Ca2+ exchanger, 5507-I-AP,
Proteintech, USA) were determined by western blotting as our previous
study 13.