Antioxidant Activity Analysis
The DPPH• (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay (RSA) was performed according to Sevim et al. (2013). The samples were measured with a spectrophotometer at 517 nm (Shimadzu Spectrophotometer UV- 1700 PharmaSpec, Japan). The trolox equivalent (TE) of the DPPH• RSA was calculated against the standard curve prepared with known concentrations of Trolox. The data are expressed as µmol TE of 100 g of each sample (R2 = 0.994) (Carrasco-Pancorbo et al., 2005).