Clinical samples
A total of 93 blood samples were collected from patients with acute coronary syndrome (ACS/PCI), a high risk of ischemia or a high risk of bleeding at Affiliated Hospital of Xiamen University and store at -20 °C. As controls, oropharyngeal swab samples from 5 healthy individuals and saliva samples of 5 healthy individuals were used.
DNA was extracted from the blood samples, oropharyngeal swab samples and saliva samples using a sample release agent produced by BioDetect (Xiamen) Biotechnology Co., Ltd. For blood, the sample and release agent are used at a ratio of 1:30; for oropharyngeal and saliva, the samples are mixed with the release agent at a ratio of 1:1. The extraction was completed by mixing and shaking for 5 s, and the DNA extracted was used for RPCR-MC, with verification by PCR-Sanger sequencing.