Clinical samples
A total of 93 blood samples were collected from patients with acute
coronary syndrome (ACS/PCI), a high risk of ischemia or a high risk of
bleeding at Affiliated Hospital of Xiamen University and store at -20
°C. As controls, oropharyngeal swab samples from 5 healthy individuals
and saliva samples of 5 healthy individuals were used.
DNA was extracted from the blood samples, oropharyngeal swab samples and
saliva samples using a sample release agent produced by BioDetect
(Xiamen) Biotechnology Co., Ltd. For blood, the sample and release agent
are used at a ratio of 1:30; for oropharyngeal and saliva, the samples
are mixed with the release agent at a ratio of 1:1. The extraction was
completed by mixing and shaking for 5 s, and the DNA extracted was used
for RPCR-MC, with verification by PCR-Sanger sequencing.