Allplex SARS-CoV-2 assay
This assay is a conventional RT-PCR assay for detecting SARS-CoV-2; it targets the E gene, nucleocapsid (N ) gene, RNA-dependent RNA polymerase (RdRP ) region of the orf1ab gene, and spike (S ) gene, with the latter two detected on the same fluorescence channel. The RT-PCR test was performed in the Hanyang University Guri Hospital or Seegene Medical Foundation (Seoul, Korea) laboratory. Nucleic acid extraction for Allplex SARS-CoV-2 assay was performed using Seeprep 32 (Seegene Inc., Korea) at the Hanyang University Guri Hospital or MagNA Pure 96 (Roche, Switzerland) at Seegene medical foundation according to the manufacturer’s instructions. Amplification and detection were performed on a Bio-Rad CFX96 thermocycler (BioRad Laboratories, The Netherlands). The results were interpreted using Seegene Viewer data analysis software. When the Ct values of all three fluorescence channels (E , N , and RdRP /Sgenes) were within the cutoff of 40.0 cycle, the result was considered positive.4