Figure 4 . Bio-energetic profile, apoptosis and
telomere length of Treg expanded in the presence of IL-7. (A )
Mitochondrial mass and (B ) mitochondrial Δψm of Treg expanded
with the StM or the IL-7M. (C ) Dynamic measurement of 2NBDG
uptake in Treg expanded with the StM or the IL-7M (D ) Graph
shows lactate concentration (mmol/l) in the supernatant of Treg expanded
with the StM or the IL-7M (E ) Treg expanded with the StM or the
IL-7M were cultured for three weeks in medium without aCD3CD28 beads nor
cytokines. The absolute number of live cell is reported in the graph.
After three weeks, remaining Treg were re-stimulated with aCD3CD28 beads
and 100 UI rhIL-2. (F ) Representative FACS plot (left) of
annexin V and propidium iodide staining in Treg expanded with the StM or
the IL-7M and cultured with an agonistic anti-fas monoclonal antibody.
Graph representing four independent experiments is shown on the right.
(G ) Representative FACS plot (left) showing Bcl-2 expression in
Treg expanded with the StM or the IL-7M and in naïve Treg after sorting
(n=3). (H ) Telomere length measure by flow cytometry using a
PNAC3TA2 probe was measured in Treg
expanded with the StM or the IL-7M and in naïve Treg after sorting
(n=4).