Figure 1. Identification and responsiveness to IL-7 of
circulating Treg subsets. (A ) Representative FACS plots
showing the gating strategy to identify Treg
(CD4+CD25highFOXP3+CD127low)
and naïve and memory subsets based on the expression of CD62L, CD45RA
and CD95. (B ) Graph shows percentage of each of Treg subset
from 16 subjects (C ) Representative FACS plot (left) showing
the expression of CD127 in Treg subsets compared to conventional CD4+ T
cells and B cells, and graph representing data from 6 subjects (right).
(D ). Representative FACS plot (left) showing STAT-5
phosphorylation in Treg subsets compared to conventional CD4+ T cells
and B cells after 10 minutes incubation with 10ng/ml of rh-IL-7 (left)
measurements from 6 subjects (right). (E ) Expansion curve of
bulk Treg cultured with medium (Ctr), IL-2 (100 UI/ml), IL-7 (10ng/ml),
and combination of IL-2 and IL-7. (n=3).