Figure 4 . Bio-energetic profile, apoptosis and telomere length of Treg expanded in the presence of IL-7. (A ) Mitochondrial mass and (B ) mitochondrial Δψm of Treg expanded with the StM or the IL-7M. (C ) Dynamic measurement of 2NBDG uptake in Treg expanded with the StM or the IL-7M (D ) Graph shows lactate concentration (mmol/l) in the supernatant of Treg expanded with the StM or the IL-7M (E ) Treg expanded with the StM or the IL-7M were cultured for three weeks in medium without aCD3CD28 beads nor cytokines. The absolute number of live cell is reported in the graph. After three weeks, remaining Treg were re-stimulated with aCD3CD28 beads and 100 UI rhIL-2. (F ) Representative FACS plot (left) of annexin V and propidium iodide staining in Treg expanded with the StM or the IL-7M and cultured with an agonistic anti-fas monoclonal antibody. Graph representing four independent experiments is shown on the right. (G ) Representative FACS plot (left) showing Bcl-2 expression in Treg expanded with the StM or the IL-7M and in naïve Treg after sorting (n=3). (H ) Telomere length measure by flow cytometry using a PNAC3TA2 probe was measured in Treg expanded with the StM or the IL-7M and in naïve Treg after sorting (n=4).