2.1 Study Design and Sample Collection
Peripheral venous blood was collected from 40 donors, all of them were
vaccinated with double dose of inactivated SARS-CoV2 vaccine and out of
40 donors, 20 donors experienced breakthrough infection during delta
wave (convalescent vaccines), the rest 20 donors did not have any prior
history of SARS-CoV2 infection or did not have any breakthrough
infection (naïve vaccinees). The blood collection was performed after
4-6 months of the completion of the second dose. To understand the
immune response after SARS-CoV2 infection blood was collected from 20
convalescent patients at least after 1 month of their testing negative
of SARS-CoV2. The details of the donors are given in Table 1. These
convalescent patients were also vaccinated and tested positive during
the delta wave of the SARS-CoV2 virus. The details of the sequence
deposited at the time of infection is available at the following link
https://inda.rcb.ac.in/insacog/statreportzonelineagegraph. PBMC
was isolated from blood, resuspended in CS10 media (Stem cell
Technologies), and stored in liquid nitrogen. Plasma was stored at -80℃
till further use.
2.2 Institutional Review Board Statement: The study was
conducted according to the guidelines of the Declaration of Helsinki and
approved by the Institutional Human Ethics Committee, Institute of Life
Sciences. The Institutional Ethics Committee (IEC)/Institutional Review
Board (IRB) reference number is 106/HEC/2021.
Informed Consent Statement: Informed consent was obtained from
all subjects involved in the study.