Cytosol preparation of rat liver tissues
Immediately after animal sacrifice the liver tissue were cleaned in phosphate buffer and homogenized (30 % w/v) in ice-cold phosphate buffer (0.1 mol /L, pH 7.4) then centrifuged at 10,000 rpm at 4ºC for 30min. The supernatant (cytosol) was collected and stored at -20˙C in different aliquots for further assays.