2.7. Measurement of body weight and thyroid weight
Body weight was recorded on the beginning, sixth, eleventh, and
fifteenth days of the trial. Thyroid weight was assessed following
exsanguinations. An electronic weighing balance (Sartorius BP 210S) was
used to measure thyroid weight.
2.8. Tissue
homogenization
The thyroid gland was removed for biochemical testing, cleaned with PBS
solution, and kept at -20oC until needed. The thyroid
gland extract was made with homogenization in 0.02 M Tris-Cl (pH 7.4).
After centrifuging homogenate tissue at 12500 rpm for 30 min at 4°C, the
supernatant was kept at -20°C for the biochemical tests.