3.3. Effect of UVB irradiation on enzymatic and non-enzymatic assays
LPO estimation- Thyroid MDA levels in the UVB-irradiated group were considerably higher than those in the control group. When compared to the UVB-irradiated group, the distribution of Naringin to the UVB+NG treated group resulted in a considerable drop in MDA levels (Fig.4.A).
SOD estimation- In comparison to the control group, the UVB-irradiated group’s SOD activity was significantly higher. When Naringin was administered to the UVB+NG treated group, the activity of SOD was markedly reduced in comparison to the UVB irradiated group (Fig.4.B).
Catalase activity- The UVB-irradiated group had significantly more catalase activity than the control group. The endogenous accumulation of hazardous H2O2 brought on by the lack of catalase activity is predicted to cause a multitude of genetic modifications, including single-strand and double-strand breaks, which may eventually result in the pathological illness. The catalase level was found to be lower in the UVB+NG treated group than it was in the UVB irradiated group after naringin administration (Fig.4.C).
H2O2 activity- When compared to the control group, the H2O2 activity seen in the UVB-irradiated group was significantly higher. When naringin was administered to the UVB+NG treated group, the activity of H2O2 was markedly reduced in comparison to the UVB irradiated group (Fig.4.D).
NO activity- NO activity was substantially higher in the UVB-irradiated group than it was in the control group. When Naringin was administered to the UVB+NG treated group, it was observed that the NO level was lower than in the UVB alone group (Fig.4.E).
GR activity- In comparison to the control group, the GR activity was significantly higher in the UVB-irradiated group. When Naringin was administered to the UVB+NG treated group, the activity of GR was markedly reduced in comparison to the UVB irradiated group (Fig.4.F).
GSH activity- When compared to the control group, the GSH activity seen in the UVB-irradiated group was significantly lower. When naringin was administered to the UVB+NG treated group, the activity of GSH was markedly increased in comparison to the UVB irradiated group (Fig.4.G).
Changes in the ROS level- Reactive oxygen species is commonly employed as a marker to detect tissue injury in toxicological and clinical chemistry. Variations in ROS level are a sign of antioxidant enzymes alterations occurring in the afflicted tissue. In comparison to the control group, the ROS activity was significantly higher in the UVB-irradiated group. When Naringin was administered to the UVB+NG treated group, the activity of ROS was markedly reduced in comparison to the UVB irradiated group (Fig.4.H).