Figure 3 Forced swimming test
Note: *P <0.05 and **P <0.01 compared with the CON+NS group; #P <0.05 and##P <0.01 compared with the MS+NS group.
3.2 Laboratory testing results
3.2.1 Effect of probiotics on neurons in CA1 area of hippocampus in mother-infant isolated offspring of depressed-like rats
The morphological and quantitative changes of neurons in the CA1 region of the hippocampus were detected using Nissler staining. Nissler staining results showed that, in terms of the number of neurons, the number of neurons in the CA1 area of hippocampus was reduced in the MS+NS group of rats compared with the CON+NS group, and the difference was statistically significant (P <0.05)ï¼›After the intervention, the number of neurons in the hippocampal CA1 area of rats in the MS+P group increased compared with the MS+NS group, and the difference was statistically significant (P <0.05); the number of neurons in the hippocampal CA1 area of rats in the CON+NS group increased compared with the MS+P group (P >0.05). Morphologically, in the hippocampal CA1 area, neurons in the CON+NS group were neatly arranged, with intact cell structure and clear borders, and abundant intracellular niche; compared with the CON+NS group, the cells in the hippocampal CA1 area of the MS+NS group showed a disordered and sparse arrangement, the number of niche in the cytoplasm was reduced, the nucleus showed solidification, fragmentation, and lysis, and the neuron decreased in number;After the intervention, compared with the MS+NS group, the neuronal cells in the CA1 region of the hippocampus of the MS+P group were more neatly arranged, the clarity of the nuclei was improved, the Nisus vesicles were increased, the staining was relatively uniform, and the number of neurons was increased. Figures 4 and 5.