Figure 8 Expression of 5-HT,cAMP,
CREB ,BDNFproteins in hippocampus of mice in each group (DAB staining,
40X)
(2) To further investigate whether probiotics activate the cAMP/CREB
signaling pathway in the hippocampal tissue of mother-infantisolated
rats, therefore, key molecules in the signaling pathway wereselected for
detection in this experiment. WB technique showed thatthe cAMP, CREB,
and BDNF protein expression levels were decreased in the MS+NS group
compared with the CON+NS group, and the differences were statistically
significant (P <0.01), compared with MS+NS group, cAMP
protein expression level was increased in MS+P group, and the difference
was statistically significant (P <0.01), CREB and BDNF
protein expression was also increased (P >0.05),
compared with CON+NS group, cAMP, CREB and BDNF protein expression level
was decreased in MS+P group, and the difference was statistically
significant (P <0.01, P <0.05,P <0.01). Figure 9.