Figure 8 Expression of 5-HT,cAMP, CREB ,BDNFproteins in hippocampus of mice in each group (DAB staining, 40X)
(2) To further investigate whether probiotics activate the cAMP/CREB signaling pathway in the hippocampal tissue of mother-infantisolated rats, therefore, key molecules in the signaling pathway wereselected for detection in this experiment. WB technique showed thatthe cAMP, CREB, and BDNF protein expression levels were decreased in the MS+NS group compared with the CON+NS group, and the differences were statistically significant (P <0.01), compared with MS+NS group, cAMP protein expression level was increased in MS+P group, and the difference was statistically significant (P <0.01), CREB and BDNF protein expression was also increased (P >0.05), compared with CON+NS group, cAMP, CREB and BDNF protein expression level was decreased in MS+P group, and the difference was statistically significant (P <0.01, P <0.05,P <0.01). Figure 9.