Figure 3 Forced swimming test
Note: *P <0.05 and **P <0.01 compared
with the CON+NS group; #P <0.05 and##P <0.01 compared with the MS+NS
group.
3.2 Laboratory testing results
3.2.1 Effect of probiotics on neurons in CA1 area of hippocampus in
mother-infant isolated offspring of depressed-like rats
The morphological and quantitative changes of neurons in the CA1 region
of the hippocampus were detected using Nissler staining. Nissler
staining results showed that, in terms of the number of neurons, the
number of neurons in the CA1 area of hippocampus was reduced in the
MS+NS group of rats compared with the CON+NS group, and the difference
was statistically significant (P <0.05)ï¼›After the
intervention, the number of neurons in the hippocampal CA1 area of rats
in the MS+P group increased compared with the MS+NS group, and the
difference was statistically significant (P <0.05); the
number of neurons in the hippocampal CA1 area of rats in the CON+NS
group increased compared with the MS+P group
(P >0.05). Morphologically, in the hippocampal CA1
area, neurons in the CON+NS group were neatly arranged, with intact cell
structure and clear borders, and abundant intracellular niche; compared
with the CON+NS group, the cells in the hippocampal CA1 area of the
MS+NS group showed a disordered and sparse arrangement, the number of
niche in the cytoplasm was reduced, the nucleus showed solidification,
fragmentation, and lysis, and the neuron decreased in number;After the
intervention, compared with the MS+NS group, the neuronal cells in the
CA1 region of the hippocampus of the MS+P group were more neatly
arranged, the clarity of the nuclei was improved, the Nisus vesicles
were increased, the staining was relatively uniform, and the number of
neurons was increased. Figures 4 and 5.