\documentclass[10pt]{article}

\usepackage{fullpage}
\usepackage{setspace}
\usepackage{parskip}
\usepackage{titlesec}
\usepackage[section]{placeins}
\usepackage{xcolor}
\usepackage{breakcites}
\usepackage{lineno}
\usepackage{hyphenat}





\PassOptionsToPackage{hyphens}{url}
\usepackage[colorlinks = true,
            linkcolor = blue,
            urlcolor  = blue,
            citecolor = blue,
            anchorcolor = blue]{hyperref}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@combinedblfloats{\box\@outputbox}{\unvbox\@outputbox}{}{%
  \errmessage{\noexpand\@combinedblfloats could not be patched}%
}%
\makeatother


\usepackage{natbib}




\renewenvironment{abstract}
  {{\bfseries\noindent{\abstractname}\par\nobreak}\footnotesize}
  {\bigskip}

\titlespacing{\section}{0pt}{*3}{*1}
\titlespacing{\subsection}{0pt}{*2}{*0.5}
\titlespacing{\subsubsection}{0pt}{*1.5}{0pt}


\usepackage{authblk}


\usepackage{graphicx}
\usepackage[space]{grffile}
\usepackage{latexsym}
\usepackage{textcomp}
\usepackage{longtable}
\usepackage{tabulary}
\usepackage{booktabs,array,multirow}
\usepackage{amsfonts,amsmath,amssymb}
\providecommand\citet{\cite}
\providecommand\citep{\cite}
\providecommand\citealt{\cite}
% You can conditionalize code for latexml or normal latex using this.
\newif\iflatexml\latexmlfalse
\providecommand{\tightlist}{\setlength{\itemsep}{0pt}\setlength{\parskip}{0pt}}%

\AtBeginDocument{\DeclareGraphicsExtensions{.pdf,.PDF,.eps,.EPS,.png,.PNG,.tif,.TIF,.jpg,.JPG,.jpeg,.JPEG}}

\usepackage[utf8]{inputenc}
\usepackage[ngerman,english]{babel}



\usepackage{float}








\begin{document}

\title{Advancing rare cancer research by MALDI mass spectrometry imaging:
Applications, challenges and future perspectives in sarcoma}



\author[1]{Maren Stillger}%
\author[1]{Mujia Li}%
\author[2]{Pia Hönscheid}%
\author[3]{Cläre von Neubeck}%
\author[1]{Melanie Föll}%
\affil[1]{University Medical Center Freiburg Institute of Pathology}%
\affil[2]{University Hospital Carl Gustav Carus}%
\affil[3]{University Hospital Essen}%


\vspace{-1em}



  
  \date{June 14, 2024}


\begingroup
\let\center\flushleft
\let\endcenter\endflushleft
\maketitle
\endgroup





\selectlanguage{english}
\begin{abstract}
MALDI mass spectrometry imaging (MALDI imaging) is uniquely suited to
advance cancer research by measuring spatial distribution of endogenous
and exogenous molecules directly from thin tissue sections. These
molecular maps provide valuable insights into various aspects of basic
and translational cancer research, including spatial tumor and tumor
microenvironment biology, pharmacological interventions, and patient
stratification. However, despite these advantages, the utilization of
MALDI imaging in studying rare cancers, which comprise approximately
20\% of all cancers, remains limited. Rare cancers pose unique
challenges in medical research, resulting in understudied entities with
suboptimal management and outcomes. In this review, we explore the value
of MALDI imaging in sarcoma, as an example of a highly heterogeneous and
challenging rare cancer. We summarize existing MALDI imaging studies in
sarcoma and outline potential future applications. In addition, we
address the specific challenges encountered when employing MALDI imaging
to rare cancers, and propose solutions, including the utilization of
formalin-fixed paraffin-embedded tissues, multi-site studies,
implementation of multiplexed experiments, and considerations for data
sharing practices. Through this review, we aim to inspire collaboration
between MALDI imaging researchers and clinical colleagues, to deploy the
unique capabilities of MALDI imaging in rare cancer research,
particularly in the context of sarcoma.%
\end{abstract}%



\sloppy


\textbf{Hosted file}

\verb`manuscript.docx`  available at \url{https://authorea.com/users/628120/articles/648844-advancing-rare-cancer-research-by-maldi-mass-spectrometry-imaging-applications-challenges-and-future-perspectives-in-sarcoma}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Figure1/Figure1}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/FIGURE2/FIGURE2}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/FIGURE3/FIGURE3}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/FIGURE4/FIGURE4}

\end{center}
\end{figure}

\selectlanguage{english}
\FloatBarrier
\end{document}

