2.4.6. Fourier Transform Infrared (FTIR) Analysis
The FTIR analysis was conducted at the Center for Grain and Animal Health Research (USDA-ARS, Manhattan, KS, USA). Attenuated total reflectance (ATR)-FTIR spectra collected at 400–4000 cm-1 range with a resolution of 4 cm-1, data spacing of 0.482 cm-1, and 32 scans using a Thermo Nicolet iS50 FTIR (Thermo Fisher Scientific Co., Waltham, MA) spectrometer equipped with a single-bounce diamond crystal and a deuterated triglycine sulfate detector. Measurements were repeated twice for each sample at ambient temperature. The quantitative analysis was done by “peak fitting” procedure and individual component bands according to a Gaussian curve fit (GRAMS/AI™ Spectroscopy Software Suite, Thermo Scientific™, Waltham, MA, USA).