2.4.2. Total Protein Content
The total protein content was analyzed with the Dumas technique at the
Soil Testing Laboratory, Kansas State University. The oleoresins (0.15
±0.01 g) were combusted, and the nitrogen content was measured on weight
percent basis by using a LECO TruSpec CN Combustion Analyzer (TrueSpec
Micro, LECO, St. Joseph, MI, USA). EDTA was used as the calibration
protein, and the total protein content of samples was calculated by
multiplying the total combustion nitrogen with a factor of 6.25.