Table 1: Comparison of IFA titres with standard heat and heat/detergent inactivation.
Heat onlya Heat onlya Heat onlya Detergent + heatb Detergent + heatb Detergent + heatb
Sample Titre IgM Titre IgG PF/IBc Titre IgMd Titre IgG PF/IB Commente
PF1 240 3840 PF 240 15,360 PF comparable
PF2 60 3840 PF 240 15,360 PF comparable
PF2 neg 3840 PF neg 3840 PF identical
PF4 neg 3840 PF neg 3840 PF identical
PF5 neg 3840 PF neg 3840 PF identical
IB1 240 960 PF/IB 60 960 PF/IB comparable
IB2 n.d. 240 IB n.d. 240 IB identical
IB3 n.d. 240 IB n.d. 240 IB identical
IB4 n.d. 240 IB n.d. 240 IB identical
IB5 n.d. 3840 IB n.d. 3840 IB identical
a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step a30min 56 °C b0.5 % Triton/0.5 % Tween/30 min 56 °C (p3) cPF = fluorescence of cytoplasm, IB = fluorescence of inclusion bodies dn.d. = not detected ecomparable titre = titre with a single dilution step deviation, identical titre = same dilution step