To further determine whether the serum inactivation protocol detrimentally impacts the ELISA, a small panel of nine sera, both native and inactivated, was tested with p3 in two dilutions (1:100 and 1:1000 for native sera and 1:33 and 1:333 for inactivated sera) by ELISA. Results for both native and inactivated sera were congruent (supporting figure 2), indicating that inactivation slightly lowers the overall antibody reactivity by a factor of 3. However, identical results could be obtained by adjusting the dilution factor, thus proving that a safe protocol for antibody detection from viraemic sera could be established.