Sample collection
The São Francisco River Basin harbors at least 205 fish species (Alves
et al., 2007), making it an excellent challenge for testing the
sensibility and resolution of different markers within such complex
Neotropical ichthyofauna. Thirty ichthyoplankton samples were used,
denoted SF01 to SF30, 22 composed of eggs and eight composed of larvae.
The larvae samples were assembled with similar-sized fragments of all
specimens to minimize bias due to larvae size and relative read
abundance estimative of input DNA from each pool.