Library preparation and DNA sequencing
According to the manufacturer’s protocol, one library for each marker was prepared using the Collibri™ PCR-free Kit (Thermo Fisher). The libraries were quantified at the start of the preparation, after each major step, and at the end, by fluorometry. The 12S libraries were sequenced in a MiniSeq™ Sequencing System (Illumina) using a MiniSeq Mid Output Kit (300-cycle), and the COI library was sequenced in a MiSeq™ Sequencing System (Illumina) using a MiSeq Reagent Kit v3 (600-cycle).