Data Analysis
We calculated mean H3 acetylation by averaging the replicates and controlling for total protein concentration. We evaluated the repeatability of the raw results by calculating the coefficient of variation (CV ) among replicates for each individual within tissues. We used the CV estimates to define outliers and performed all subsequent tests on a dataset that excluded the most divergent replicate from any replicate sets with CV of 25% or higher. We also calculated CV among individuals for each tissue, before and after removing outliers. We tested for differences in H3 acetylation mean and variance between liver and spleen by t-testsand f-tests . We also tested for differences in H3 acetylation levels in both tissues between individuals with high and low epigenetic potential. Additionally, we compared H3 acetylation to TLR-4expression using Pearson’s correlation for both liver and spleen tissues.