Data Analysis
We calculated mean H3 acetylation by averaging the replicates and
controlling for total protein concentration. We evaluated the
repeatability of the raw results by calculating the coefficient of
variation (CV ) among replicates for each individual within
tissues. We used the CV estimates to define outliers and
performed all subsequent tests on a dataset that excluded the most
divergent replicate from any replicate sets with CV of 25% or
higher. We also calculated CV among individuals for each tissue,
before and after removing outliers. We tested for differences in H3
acetylation mean and variance between liver and spleen by t-testsand f-tests . We also tested for differences in H3 acetylation
levels in both tissues between individuals with high and low epigenetic
potential. Additionally, we compared H3 acetylation to TLR-4expression using Pearson’s correlation for both liver and spleen
tissues.