Steps Temperature Time Cycles
Initial denaturation 95οC 15min
Denaturation 94oC 30sec
Annealing 60oC-50oC 45sec 19
Extension 72οC 60sec
Denaturation 94οC 30sec
Annealing 50oC 45sec 20
Extension 72oC 60sec
Final Extension 72oC 20min