not-yet-known not-yet-known not-yet-known unknown Microsatellite amplification Eight microsatellite loci were analyzed by multiplexing in two independent reactions (four loci per reaction – Table S2 _ Appendix). Multiplex PCRs were performed in 10 μl reactions using 5 μl of Qiagen Buffer from the Qiagen multiplex PCR kit and 30 ng of genomic DNA. Primer concentration for each amplified locus is given in Table S2 (Appendix). PCR amplification was performed using a TD 60-50 protocol that was set according to Table S3 (Appendix). Microsatellite analysis was performed in an ABI 3130l Genetic Analyzer (Applied Biosystems®) and genotypic data were obtained using the Geneious R.v11 software.