RNA Extraction, Library Construction, and Sequencing
We extracted total RNA from the collected samples after sample lyophilization using ReliaPrepTM RNA Tissue Miniprep System (Promega Corporation) following manufacturer protocol. The quantity and quality of the extracted RNA were assessed using a Qubit (Thermo Scientific), while RNA integrity was confirmed by 1 % (w/v) agarose gel electrophoresis and by a Bioanalyzer 2100 (Agilent Technologies) using the RNA 6000 Nano LabChip Kit. RNA samples with RIN ≥ 6 were considered suitable for sequencing.
Libraries for RNA sequencing were constructed using the TruSeq Stranded mRNA Sample Prep LT Protocol (Illumina), and were sequenced with 2x100 bp paired-end reads on an Illumina HiSeq2500 platform, using a HiSeq Flow Cell v4 with HiSeq SBS Kit v4 (Illumina) at the Centro de Genômica Funcional Aplicada à Agropecuária e Agroenergia, ESALQ, USP, Brazil.