The effect of MnSO4 concentration and selection
line on behavior
Activity was a repeatable behavioral trait (Pearson’s correlation
coefficient=0.437; P =0.002). There was no significant interaction
between selection line and concentration neither concentration affected
fish activity (Figure 2A, Table S2). Large-selected fish had overall
higher activity levels than small-selected fish (t=2.633, P < 0.01; Figure 2A, Table S2) and larger fish had higher
activity levels irrespective of the selection line (t=2.988,P =0.006; Table S2).
Among small- and large-selected fish, 22% and 9% of the fish,
respectively, did not feed during the feeding trial. The interaction
between the selection line and concentration explained differences in
feeding probability (z=1.580, P =0.002; Table S2). While among
large-selected fish the feeding probability increased with
MnSO4 concentration, among small-selected fish the
feeding probability slightly increased first but then decreased
distinctively in the highest concentration (Figure 2B). Fish body size
did not affect feeding probability (Table S2).