Abstract
The use of a combination of
several antibacterial agents for therapy holds great promise in reducing
the dosage and side effects of these agents, improving their efficiency,
and inducing potential synergistic therapeutic effects. Herein,
R12-AgNPs were produced with the supernatant of an ionizing
radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by
one-step under room temperature. The biosynthesized AgNPs presented
fascinating antibacterial activity and peroxidase-like properties, which
endowed it with the capability to catalyze the decomposition of
H2O2 to generate hydroxyl radical. After
combination of R12-AgNPs and H2O2, an
excellent synergistic bacteriostatic activity was observed for bothE. coli and S. aureus , especially at low concentrations.
In addition, in vitro cytotoxicity tests showed R12-AgNPs had good
biocompatibility. Thus, this work presents a novel antibacterial agent
that exhibits favorable synergistic antibacterial activity and low
toxicity, without the use of antibiotics or a complicated synthesis
process.
Keyword: AgNPs; Deinococcus wulumuqiensis ;
Peroxidase-like activity; Antibacterial activity; Combination therapy;
Synergistic antibacterial activity
Graphical Abstract