Fig.7 Cell viability of R12-AgNPs after 24 h incubation at different
concentrations.
Conclusion
In conclusion, the AgNPs synthesized using the cell-free supernatant ofD. wulumuqiensis R12 exhibit several advantageous properties,
including peroxidase-like activity, strong bacteriostatic capabilities,
and excellent biocompatibility. These AgNPs effectively deactivate
bacteria by generating toxic ROS that disrupt bacterial membranes.
Moreover, the intrinsic peroxidase-like activity of R12-AgNPs enables
the catalysis of H2O2 decomposition,
producing hydroxyl radicals at low concentrations and enhancing
synergistic antimicrobial effects while maintaining biosafety. Through
the combination with H2O2, R12-AgNPs
have demonstrated exceptional effectiveness in antimicrobial therapy.
This approach holds promise as a candidate for antibiotic-free treatment
of bacterial infections. The combination of R12-AgNPs and
H2O2 presents a novel strategy that
could address the growing concern of antibiotic resistance and offer a
safer and effective alternative for combatting bacterial infections.