RNA extraction and real-time quantitative polymerase chain reaction
RNA lysate RZ is used to homogenize mice colons, followed by RNA extraction using the RNAsimple total RNA kit. Reverse transcription is carried out with HifairTM Ⅱ 1st strand cDNA synthesis supermix for qPCR, and real-time quantitative PCR is performed with SYBR Green Realtime PCR Master Mix on an Applied Biosystems 7500 Fast Real-Time PCR system. PCR amplification is performed with the primers listed in Table 2.
Table 2. Sequences of primers.