2.5 DNA extraction and polymerase chain reaction amplification
Total DNA was extracted from mouse feces using the PowerSoil® DNA
Isolation kit according to the manufacturer’s instructions. After
extraction, the 16S full-length primer was designed according to the
conservative region 27F-1492R, for polymerase chain reaction (PCR)
amplification of the target area (10μL system, Solexa PCR). The
synthesized specific primers (27F-1492R) were designed as follows:
Forward primer 27F: AGRGTTTGATYNTGGCTCAG; Reverse primer 1492R:
TASGGHTACCTTGTTASGACTT.