Mouse clinical score after treatment.
After CLP-induced polymicrobial sepsis successfully in mice, all of them moved free and none needed analgesia for pain immediately. The clinical scores of mice at 0h, 2h, 6h, 12h, 24h, 48h, 72h, 4d, 5d and 7d by incorporating to the Murine Sepsis Score (MSS) were evaluated. The scoring criteria are as follows: spontaneous activity, response to touch and auditory stimuli, posture, respiration rate and quality (labored breathing or gasping), and appearance (i.e., degree of piloerection), each of these scores variables between 0-4[22].