Fig.7 A, apoptosis by TUNEL analysis (Original magnification: 200X Scale:100um). Representative confocal fluorescence microscopy images of spleen cells stained with antibodies against Tunnel (green) and DAPI(blue). DAPI ,4’6-diamidino-2-phenylindole. Scale bar, 20 mm.B, the percentage of apoptosis were determined average gray value by Image J. C, western-blot of apoptosis BCL-2 and Bax in spleen, D and E , Densitometric analysis of the relevant bands was performed by Image J. The values presented are mean ±SEM (n=5). *p<0.05, **p<0.01, ***p<0.001, n=5, The data are representative of 3 independent experiments.