Anti-CD28 reduced CLP-induced splenocytes apoptosis
T lymphocyte subsets differentiation and lymphocytes apoptosis played an
important role in septic rats[26, 27]. The proportion of spleen cell
apoptosis was significantly increased in CLP mice, with reduced nucleus
number, irregular shape of the nucleus, state of condensation or nuclear
fragments appear, while anti-CD28 relieved such spleen apoptosis
significantly (Fig.7a, b). The BCL-2 and Bax signaling pathways are
required in spleen homogenate for apoptosis in CLP-induced septic
mice[28]. As illustrated in Figure.7c, expression of the BCL-2 was
significantly increased after anti-CD28 challenge (Fig.7d,
p<0.01), while expression of the Bax was remarkably decreased
(Fig.7e, p<0.01).