Plasma cytokine
The patients’ serum was collected as shown above, and mice blood samples were collected by heart puncture when anesthetized by sevoflurane 3 days after CLP and CD28 challenge. Then, the mice’s blood was sent into ethylenediaminetetraacetic acid tube and placed in a centrifuge tube, centrifuge at 12,000 rpm for 5 minutes, take the supernatant, measure the content of TNF-a, IL-17, IL-10, and IL-6 in patients or mice serum according to Elisa manufacturer’s recommendations (Thermo Fisher).