Reagents
Mouse TNF-a, IL-17A, IL-10, IL-6 enzyme-linked immunosorbent assay (ELISA) kits, CD28 functional antibody, fluorescent-labeled monoclonal antibodies anti-mouse CD4-FITC, anti-mouse IgG1-FITC, anti-mouse CD25-PE, anti-mouse IgG1-PE, anti-mouse FoxP3-APC, anti-mouse IFN-gamma-PE, anti-mouse IgG1-APC, and anti-mouse IL-17-APC were bought from eBiosciences (San Jose, CA, USA). FIX & PERM medium from Invitrogen, USA. Leukocyte Activation Cocktail from BD, Catalog No.550583, contain PMA(Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin) and the protein transport inhibitor BD GolgiPlug™ (Brefeldin A).