Fig.7 A, apoptosis by TUNEL analysis (Original magnification:
200X Scale:100um). Representative confocal fluorescence microscopy
images of spleen cells stained with antibodies against Tunnel (green)
and DAPI(blue). DAPI ,4’6-diamidino-2-phenylindole. Scale bar, 20 mm.B, the percentage of apoptosis were determined average gray
value by Image J. C, western-blot of apoptosis BCL-2 and Bax in
spleen, D and E , Densitometric analysis of the
relevant bands was performed by Image J. The values presented are mean
±SEM (n=5). *p<0.05, **p<0.01, ***p<0.001,
n=5, The data are representative of 3 independent experiments.