Patient characteristic
Patients with sepsis were recruited and the study was approved by the
human ethics committee of Changhai Hospital (CHEC2019-133), as
preliminary trial of sepsis treatment(CTR20191855), informed consent
from all of the subjects or their relatives was obtained before
enrollment. 65 patients suffering from sepsis were admitted to the
intensive care unit (ICU) in Shanghai Changhai Hospital from January to
October 2020, meanwhile 13 healthy volunteers, gender, age and ethnicity
was the same, as the control group were recruited. The sepsis diagnostic
criteria according to the definitions for sepsis and septic shock
established by The Third International Consensus (Sepsis-3)[20].
Eligibility criteria for enrollment in this study included the sepsis
patients of either sex by age 18–80 years with systolic blood pressure
<80 mmHg, presenting within 8 h of sepsis after taking proper
consent from the patient or relatives. The severity of the patients was
assessed by Acute Physiology and Chronic Health Evaluation score (APACHE
Ⅱ) and the extent of development of organ dysfunction was assessed by
the Sequential Organ Failure Assessment score (SOFA; range, 0–24).
Exclusion criteria included: 1. age <18 years; 2. severe acute
head injury (GCS score <5); 3.irresuscitable terminal stage at
presentation; 4. autoimmune disease (such as rheumatoid arthritis,
systemic lupus erythematosus, asthma, or multiple sclerosis); 5. AIDS;
6. acute stroke, myocardial infarction or recent viral hepatitis;7.use
of hormones or immunosuppressors within the past 3 months before
hospitalization;8.transplant surgery;9.unexpected termination of CBP
treatment;10.Patients who resuscitated with anti-inflammatory drugs or
corticosteroids; 11. septic patients, of whom blood sampling was not
taken within the first 8 h after definition sepsis.