Anti-CD28 Ab amended Th17/Treg imbalance in sepsis mice
As Th17 and Treg variation in sepsis patients, Th17 and Treg variation in CLP induced sepsis mice had similar trends. Treg and Th17 in spleen of CLP mice were decreased obviously (Figure 6a, b). After treated with CD28 antibody, the extent of Treg and Th17’ increase amplitude was lower, as well as the proportion of Th17/Treg (Figure 6c, d, e). Serum levels of IL-6,TNF-α and IL-17A were increased significantly in CLP mice, while the level of IL-10 was increased slightly. Meanwhile, such trends were reversed by administrated with CD28 functional antibody (Fig6. F, G, H and I).