Th17 and Treg expression on CD4+T lymphocytes and their ratio were compared between sepsis patients and volunteers. Although there was no difference in health volunteers and septic patients [4.998(3.474-6.523) vs 4.625(4.117-5.133), P=0.5573], sepsis caused Th17 increased obviously [0.6977 (0.4852-0.9102)vs 3.580(3.209-3.951), P<0.01]. And the ratio of Th17 to Treg higher in sepsis patients [0.2131 (0.1400-0.2600) vs 0.7907 (0.5575-1.038), P <0.01]. This suggest in initial sepsis Th17 cells increase significantly, while Treg which acted as an inflammatory brake remained relatively stable.
Figure 1: A, Th17 expression in healthy volunteers and sepsis patients, take CD3 as abscissa and CD8 as ordinate, find out the CD3+CD8-cell group, and then mark IL-17 (ordinate). B, Treg expression in healthy volunteers and sepsis patients, take CD4 as the abscissa, find out CD4+cell group, and then mark FoxP3 (ordinate).C, Expression of Th17 between health volunteers and septic patients. D , Expression of Treg between health volunteers and septic patients. E, Expression of Th17/Treg between health volunteers and septic patients, ***, p<0.001, ****, p<0.0001.