Plasma cytokine
The patients’ serum was collected as shown above, and mice blood samples
were collected by heart puncture when anesthetized by sevoflurane 3 days
after CLP and CD28 challenge. Then, the mice’s blood was sent into
ethylenediaminetetraacetic acid tube and placed in a centrifuge tube,
centrifuge at 12,000 rpm for 5 minutes, take the supernatant, measure
the content of TNF-a, IL-17, IL-10, and IL-6 in patients or mice serum
according to Elisa manufacturer’s recommendations (Thermo Fisher).