Reagents
Mouse TNF-a, IL-17A, IL-10, IL-6 enzyme-linked immunosorbent assay
(ELISA) kits, CD28 functional antibody, fluorescent-labeled monoclonal
antibodies anti-mouse CD4-FITC, anti-mouse IgG1-FITC, anti-mouse
CD25-PE, anti-mouse IgG1-PE, anti-mouse FoxP3-APC, anti-mouse
IFN-gamma-PE, anti-mouse IgG1-APC, and anti-mouse IL-17-APC were bought
from eBiosciences (San Jose, CA, USA). FIX & PERM medium from
Invitrogen, USA. Leukocyte Activation Cocktail from BD, Catalog
No.550583, contain PMA(Phorbol 12-Myristate 13-Acetate), a calcium
ionophore (Ionomycin) and the protein transport inhibitor BD GolgiPlug™
(Brefeldin A).