Culture splenocytes
After the mice were completely anesthetized by sevoflurane, the mice were killed by cervical dislocation. Then place the mouse in a bottle filled with alcohol for 5 minutes to sterilize, and operate in a sterile biological operation table: use a sterile instrument to perform laparotomy to remove the spleen tissue of the mouse and place it into a sterile mill previously added with sterile PBS Grind gently in the grinding dish to completely separate the spleen cells, then add erythrocyte lysate and let stand for 10 minutes, then stop with sterile PBS, centrifuge to remove the lower layer of cells. After filtering out the non-cellular components with a filter, count 1×106cells into a sterile culture dish, add Leukocyte Activation Cocktail 1ul and incubate for 6 hours at 37 ° C with 5% CO2.