Fig.6 Flow cytometry of Treg cell(A ), Th17 cell(B ) in spleen after 3 days of CLP. The percentage of Treg(C ) and the Th17 (D ) in different groups by flow cytometry. E , the ratio of Th17 to Treg is calculated. Expression of serum related inflammatory factors in mice 3 days after CLP, IL-6(F ), TNF-a(G ), IL-17A(H ) and IL-10(I ). The values presented are mean ±SEM (n=5). *p<0.05, **p<0.01, ***p<0.001. The data are representative of 3 independent experiments.