2.4. Characterizations
The morphologies of the photothermal films were acquired with a Scanning
Electron Microscope (SEM, SU8200, HITACHI, Japan), and the elemental
maps and percentage were obtained by the corresponding Energy Dispersive
Spectrophotometer (EDS). The Transmission Electron Microscopy (TEM) of
samples is gained by JEM2010. X-ray diffraction patterns (XRD) of the
samples were characterized by D8 Advance, Bruker. Strain-Stress curves
were collected by Electronic Universal Material Testing Machine
(WDW-3020). Autosorb iQ2 was used to achieve
Brunauer-Emmett-Teller (BET). The water contact angle of the solar
heating membranes was measured by Dataphysics OCA25, Germany. The
Ultraviolet-Visible- near infrared (UV-vis-NIR) absorption spectroscopy
was achieved by Perkin Elmer, Lambda 1050+. The Discovery TGA was
utilized for thermogravimetric analysis (TGA). The differential scanning
calorimetry (DSC) was collected by DSC Q2000.
The photothermal data of the materials were acquired by FLIR T620 and
analyzed using the matching software FLIR Tools. Sunlight in the
laboratory is obtained through a simulated daylight xenon light source.
Solar irradiation was obtained by a TES 132 Datalogging solar power
meter. Temperature information was gained from windy.com. Location
coordinates were obtained from Google map.
CCK-8 was used to detect the changes of cell viability in the co-culture
of HSF cells and samples for 12h and 24h. HSF cells in logarithmic
growth stage were taken, inoculated into the 24-well plate according to
4×104/well, and the culture medium was incubated at 5% CO2 and 37℃ for
12h and 24h. After that, the medium was removed, and each well was
washed with PBS for three times, and incubated for 2 hours at 37℃ in a
constant temperature incubator by adding medium containing 10% CCK-8,
5% CO2 at 1mL/well. The absorbance at 450nm was detected by zymography
and the relative viability was calculated.