4. Regulation of the fidelity of translation initiation and
ribosome biogenesis
The failure of the 3GC mutant i-tRNA in initiation in vivo ,
formed the basis for our genetic screen to isolate suppressor mutations
(in the genome) that allow initiation with the 3GC mutant i-tRNA
(Fig. 2B, iv ). A detailed characterisation and molecular
analysis revealed the roles of, (i) i-tRNA gene copy number and its
abundance; (ii) one-carbon metabolism, Fmt and FolD; and (iii) ribosomal
components, ribosome biogenesis factors, translation factors and their
coevolution; in the fidelity of translation initiation. In addition, the
suppressors have provided newer insights into the process of ribosome
biogenesis/maturation. We discuss these findings in the context of
translation initiation, and ribosome biogenesis.