Reference Genome Assembly
The draft genome used in this study was 9.9 Gb in length and totalled 2,584 contigs. The longest contig was 182 Mb long and the mean contig size was 3.8 Mb. The L50 (which is the number of contigs, ordered longest-to-smallest, to comprise 50% of the assembly size) was 77, and the N50 (the size of the contig that passes the 50% assembly size threshold) was 40.2 Mb.
The maximum likelihood tree constructed from the R genes in the draft assembly was annotated according to peptide motifs that are diagnostic for either CNL or TNL identity (Figure 2). A deep evolutionary divergence between the two broad classes is seen, although we observe isolated instances of R genes falling incongruently within the wrong clade.