Library Enrichment
The libraries of seven S. integrifolium that were sequenced using WGS, and thus were unenriched for R genes, contained a median of 1.78% of reads that mapped with high fidelity (98% identity or higher) to contigs in the Pan-NLRome. Libraries that had been enriched for R genes using oligo baits designed from Helianthus annuus contained a median of 63.4% of high fidelity reads mapping to R genes, representing a 36-fold enrichment for R genes (Figure 3).