Draft Genome Assembly
A chromosome-scale genomic assembly of an F1 Silphium
integrifolium x S. perfoliatum is being prepared at the time of
writing. The S. integrifolium mother was a leaf blotch resistant,
large-headed selection from a breeding population that had undergone
approximately 6 cycles of recurrent selection for increased number of
fertile ray florets (“feminization”) per head and disease resistance.
The founders of the breeding population were sourced from wild central
Kansas populations, i.e., located within the geographic region we call
“West” in this study. Flash-frozen tissue was sent to Arizona Genomics
Institute for high-molecular-weight DNA extraction, which was then
prepared for PacBio Sequel-II CCS sequencing at the Genome Sequencing
Center at HudsonAlpha Institute for Biotechnology. The libraries were
sequenced at roughly 21.5X depth assuming a 10 Gb genome size. Reads
were assembled de novo using hifiasm assembler (Cheng et al.,
2021) using default parameters.