Figure 5. Off-site genome deamination by A3A, A3B, and AID upon HBV rcDNA depletion. Off-site mutations were measured by 3D-PCR in CpG-rich regions of (A) TP53 , (B) ARID2 , and (C) PAX5 genes. Mock, non-targeting siRNA control; LAM, treatment with lamivudine; DMSO, treatment with DMSO as a control for LAM treatment. Generation of amplicons at lower melting temperatures indicates accrual of deamination-associated mutations.