Figure 7. Effects of HBV DNA depletion on anti-HBV activity of
A3C, A3D, and A3H. Analysis of (A) HBsAg, (B)intracellular HBV DNA, and (C) cccDNA levels. (D-I)3D-PCR analysis and semi-quantitative analysis of deamination of cccDNA,
intracellular HBV DNA, and secreted HBV DNA.
At the same time, 3D-PCR analysis of the host genome did not detect
deamination in selected regions of genes in either experimental group
(Figure 8). This might be due either to lower ability of A3C, A3D, and
A3H to affect the host genome, or a different set of targets mutated by
these cytidine deaminases.