Figure 3. Effects of anti-HBV siRNA on APOBEC/AID-mediated antiviral and deaminating activity. Antiviral activity of CRISPR-activated APOBEC/AID with or without anti-HBV siRNA measured by determining levels of (A) HBsAg, (B) intracellular HBV DNA, and (C) cccDNA. HBV cccDNA deamination was analyzed by(D) 3D-PCR analysis. (E) Semi-quantitative analysis of cccDNA deamination. (F) 3D-PCR analysis of secreted HBV DNA deamination. (G) Semi-quantitative analysis of secreted HBV DNA deamination. (H) 3D-PCR results and (I)semi-quantitative analysis on intracellular HBV DNA. Mock, scrambled siRNA. NC, non-targeting sgRNA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.