HBsAg and analysis
Media that had been conditioned by cells were collected and filtered through 0.2-μm filters to eliminate any cellular debris. HBsAg was quantitated using the DS-IFAHBsAg-0.01 colorimetric ELISA, following the manufacturer’s instructions (Diagnostic Systems, Russia).

Statistical analysis

Values were expressed as the mean ± SD of triplicate experiments using SPSS software (SPSS 21.0.0.0). One-way ANOVA and Student’s t-tests with Tukey’s HSD post hoc tests were used to compare variables and calculate p values to determine statistically significant differences in means.