2.1 Culture conditions
The biological material was the IMP-BG-036 strain, corresponding to the marine dinoflagellate P. micans , which is part of the strain of the Aquatic Organisms Germplasm Bank (BGOA). The cultivation was of the batch type, for 11 days, enriched with medium FL, arranged in 250ml glass flaks and in triplicate, submitted to a temperature of 18°C, with photoperiod 12 h, according to experimental design factors detailed in Table 1.
[Table 1]
The data generated was subjected to a complete factorial experiment 23, with 3 replicates and 3 central points, completely randomized, the treatments were shown in table 2.
[Table 2]