2.4 Determination of fatty acids
The derivatization of these compounds was carried out using the method of Ichihara and Fukubayashi (2010). 0.2ml toluene, 1.5ml methanol and a mixture of 1ml concentrated hydrochloric acid with 4.3ml methanol were added to the extracted lipids and the mixture was incubated at 45°C, then FAMEs were extracted with 1.5ml hexane and 1ml of water. Varian CP-3800 Gas Chromatograph conditions were as follows (Table 3).
[Table 3]
Statistical analysis
For the evaluation of the data obtained, an analysis of variance (ANOVA) and Tukey’s post hoc test will be carried out to evaluate the value of significance (α=0.05). Likewise, for the relationship of the lipid profile and fatty acids against the crops, a design of significant effects and graphs (Pareto) will be carried out in the statistical program Minitab 16.
Results