3.1 Growth characteristics of the P. micans strain IMP-BG-036
Figure 1 shows that the cell concentration was affected by all the
factors as a whole: the volume of the inoculum, the lighting on the
culture and the dose of nitrate in the medium, without there being a
single selective factor. Although the standardized effects of the Pareto
Diagram show that the factor with the greatest impact was the volume of
the inoculum, the interaction between it and the lighting, as well as
the dose of nitrate and lighting allowed the recording of the maximum
cell concentrations at the end of the culture period on day 11. This
result (3.1x104 cel.ml-1), was
obtained with a lighting of 5
mol.m-2.sec-1, an inoculum volume of
200 ml and without nitrate dose. in the culture medium (Table 4).
[Figure 1]
[Table 4]
One aspect observed during the counting process and not considered in
the design was the number of empty shells found; with higher incidence
in crops whose inoculum was 200 mL. In those with a smaller volume of
inoculum (50 and 125 ml), the medium was more diluted, and the valves
found were very few.