not-yet-known not-yet-known not-yet-known unknown 1 Triglyceride quantification The TG quantification method used in the biochemical validation of this study was based on the liver lipid quantification methods introduced by Nahon et al. (2018)27 and de Jong et al. (2022)28. TG was extracted from liver specimens higher than 10mg. The liver specimens were homogenized using a potter machine in a solution of 200-500 µL Nonidet™ P 40 Substitute (Sigma‐Aldrich Corp., St. Louis, MO, USA), depending on the weight of each specimen. From the homogenate, 10 µL aliquots were retained for protein analyses, and the remaining homogenate was subjected to three cycles of heating in the presence of Nonidet™ P 40 Substitute until the solution became clear, followed by cooling on ice to ensure the complete solubilization of TGs. Any insoluble material was subsequently removed by centrifugation at 13,148 rcf for two minutes. The supernatant, enriched with solubilized TGs, was used for quantification. TG concentrations were determined using the same enzymatic colorimetric assay described by Out et al.29, with the absorbance measured at 490 nm and an incubation period of 30 minutes at 37°C. Standard TG samples were employed as a reference during the colorimetric assays. Protein concentrations in the liver were determined using an assay similar to the Pierce™ BCA Protein Assay Kit (ThermoFisher Diagnostics, Waltham, MA, USA). The TG contents were normalized per unit protein mass by dividing the measured TG concentrations by the corresponding protein concentrations. 1 Triglyceride quantification