not-yet-known not-yet-known not-yet-known unknown Conflict of interest The authors H.G., A.B.T., H.Z., I.P.J.A., and K.C.H. have filed a PCT (the Patent Cooperation Treaty) application, and they stand to benefit should the patent be awarded in the national phase. Number of text pages: 17 Number of tables: 2 Number of figures: 6 Lay Summary This research explored a portable ambient light-compatible spectroscopic probe to non-invasively assess global and macrovesicular steatosis in human liver specimens. Our findings suggest that this method can be a reliable tool to aid surgeons’ decision-making on a liver’s suitability for transplantation. Graphic Abstract