Immunohistochemical investigation of the upper, lower and
nictitating membrane of Elanus caeruleus
Immunohistochemical study to the upper and lower eyelids revealed that
the CD1a-positive Langerhans cells of skin are distributed in the
stratum basal more than the stratum spinosum of the squamous epithelium.
As well as, it appears in the stroma around the blood vessels, HEV and
around and inside the feather follicle. Moreover, no Langerhans cells
were observed near the conjunctiva surface.
In addition, there is a varying concentration and un-equal distribution
of lymphocytes within the CALT. Where, the B cells which cluster under
the basement membrane were higher number than T cells which scattered
close to blood vessels. While, the plasma cells were the fewest and
randomly distributed within the CALT and it increase around HEV.
In the third eyelid, population of CD20+(B cell), CD3+(T cell) and CD1a+
(Langerhans cells) increase in density around the blood vessels, where
the highest concentration was to T cells, while, the lowest population
of the plasma cells diffuse as singular cells between the two surface of
the membrane.