Histological study
Three specimens of eyelids were fixed in 10% neutral formalin for three
days, then put in 70% ethyl alcohol for two days, after which they were
dehydrated through a series of ethyl alcohols, cleared in methyl
benzoate for three days, embedded in paraffin wax, sectioned serially
(5μm) and then sections were stained with Hematoxylin and Eosin,
Masson’s trichromic stain, and periodic acid-Schiff (PAS) (Drury &
Wallington, 1980).