Scanning electron microscopy study
The eyelids of two specimens were cut into small pieces and directly
fixed in 5% glutaraldehyde in a cacodylate buffer for 48 h at 4°C and
washed in three changes of 0.1% cacodylate buffer, and then, the
specimens were post-fixed in a cacodylate-buffered solution of 1%
osmium tetroxide for 2 h at 37°C. The specimens were washed in the same
buffer three times, dehydrated through an ascending series of ethyl
alcohol, and then infiltrated with amyl acetate for 3 two days. The
drying of the specimens was accomplished by the critical point drying
using liquid carbon dioxide, mounted and sputter-coated with gold. The
specimens were examined on a Jeol scanning electron microscope (JS
M-5400IV) at 15 kv.