Fourier transform infrared spectroscopy (FTIR)
In order to recognize the surface functional molecules involved
in biosorption of As (V), FTIR spectra of normal and As treated bacteria
was studied. Infra-Red spectra of Bacillus mycoides NR-5
(control) reveals three short peaks at 3588.8, 3277.9 and 2923.7
cm-1 (wave number) representing presence of alcohol
and alkyl groups. Arsenic biosorption on the cell surface results in a a
single broad and strong peak at 3379.0 Cm-1corresponding to amine and indicated interaction of surface
protein-metal complex. In zone 5, two peaks at 1654.0
Cm-1 and 1541 Cm-1 attributed to the
amide (I and II) bands were shifted to 1652.9 and 1541.0
Cm-1 strengthening the possibility of metal
interaction with surface proteins (Figure 6 a & b).