Identification of arsenic tolerant NR5
Based on arsenic and multiple metal resistance evaluation, NR5 was found
the best candidate for further screening and experimentation of arsenic
removal study in vitro and in vivo . The colony
characteristics of NR5 was rhizoid, large, flat, non-pigmented colonies
with filiform margins (Figure 1a). Gram staining described the bacteria
as Gram positive strain. Genomic DNA isolated from NR5 was visualized on
1% agarose gel electrophoresis. Spectroscopic estimation results DNA
yield as 250ng/µl. A260/A280 and A260/A230 ratio were 1.78 and 1.29
respectively, and results confirmed of no RNA and protein contamination
in extracted DNA. The gDNA was also free from phenolic contamination.
Sequence data obtained from ABI Sanger sequencer was subjected to
quality check. Peaks in the data were sharp with no noise, and there was
no chimeric sequence found in the sequences. EzTaxon represents that
submitted sequences were belonging to Bacillus mycoides and thus
NR5 labeled as Bacillus mycoides NR5 (Figure 3). The sequence was
deposited in GenBank with accession number KX268123. The culture was
submitted to National Depository ICAR-NAIMCC, Mau, India, with the
accession number NAIMCC-B-02103.