Survey and collection of samples from heavy metal
contaminated sites
After a thorough survey of heavy metal contaminated soil, the bulk
soil/rhizosphere soil of plants growing on metal-contaminated sites
along with water samples from polluted water-bodies were collected from
different locations of Jharkhand, Wardha; Nagpur, Maharashtra, India and
Bhanpura, Bhopal (MP). All the samples were collected in sterilized
polybags and tubes, and transported and stored in laboratory at 4℃.
Samples were analysed for their pH level and the heavy metal content was
estimated using ICP-OES (Perkin Elmer, USA).