In vitro evaluation of Antibiotic resistance and plant
growth promoting traits in NR5
B. mycoides NR5 was tested against 20 antibiotics and was found
resistant to 16 antibiotics. NR5 was found to be highly resistant
recorded against Amikacin, Ciprofloxacin, Co-Trimoxazole, Erythromycin,
Gentamicin, Nalidixic Acid, Netillin, Nitrofurantoin, Norfloxacin, and
Tobramycin, moderately resistance to Cefadroxil, Ceftriaxone,
Cloxacillin, and Vancomycin, and low resistance to Cefoperazone and
Ceftazidime. There was no growth inhibition in Ampicillin, Amoxycillin
Chloramphenicol and Penicillin (Table S2).
B. mycoides NR5 was evaluated for having plant growth promoting
attributes such as phosphate solubilization, IAA production,
siderophore, ammonia production and nitrate reduction. The qualitative
test for P-solubilization was found positive, and the subsequent
quantitative estimation of B. mycoides NR5 was calculated as
115.51±0.47 µgL-1. The NR5 was also found to possess
siderophore production ability and the index for production of
siderophore was calculated to be 28.61% unit (Table S3). Similarly,
ammonia production, which is also an important PGP trait, was also high
and the bacterium was a good ammonia producer. NR5 was also found
positive for nitrate reduction assay but the efficiency was not high.