Amplification of arsenic reductase gene (arsC) in Bacillus mycoides NR5 indicating partial contribution in arsenic tolerance mechanisms of bacterium. Primer (arsC1) designed using arsenic reductase gene sequences amplified 202bp amplicon. The amplified product was sequenced and analyzed which shared homology to arsenic reductase. The nucleotide sequence was submitted to Genbank with Accession No OP086227.