Evaluation of application of B. mycoides NR5 on
alleviation As toxicity in Spinach (Spinacia oleracea L.)
The effect of arsenic (V) on spinach plant was studied using pot
experiment. Sandy loam textured soil was collected from non-contaminated
fields, air dried, sieved through a 2mm mesh, homogenized and a total of
3.0 kg of air-dried sterilized soil was placed in each pot. As 25mg
Kg-1 supplied in the form of Sodium
arsenate dibasic heptahydrate (Na2HAsO4.7H2O) was
mixed with soil and kept for a week to stabilize the mixture. Plants
without arsenic served as control. Pot experiment was performed
in Completely Randomized Design (CRD) in three replicates. Seeds of spinach (Spinacia oleracea L.) var . F1-MULAYAM
were disinfected with 1% sodium hypochlorite solution, washed with
deionized water three times to completely remove disinfectant and then
placed in Petri dishes inside the folds of wet filter papers at 25±1ºC
for germinated. Four days later germinated seeds were withdrawal from
wet paper towel and coated with 1% carboxy methyl cellulose (CMC) and
air dried. Five coated seeds were placed in each pot and a control, and
marked properly. This experiment was carried out in controlled
conditions. The position of pots was changed on a regular basis to
ensure similar light and temperature exposure. The plants were irrigated
with deionized water (100 mL) twice per day and one set (from each
concentration) of pot harvested after maturation at 45, 60, and 75 days
after inoculation. The plants were washed with tap water and then
deionized water and were cut down into shoots and roots. Fresh weights
as well as length and shoot diameters and other physical appearance were
recorded. Roots and shoots were oven dried at 70 ºC for 48 h, powdered
and sieved through 2 mm mesh size.