Author Contributions
Renu Shukla conceptualized and supervised the experiment, Khan Mohd Sarim, Manish S. Bhoyar performed the experiment, Khan Mohd Sarim analyzed the data. Khan Mohd Sarim and Renu Shukla, and Dhanjay P. Singh wrote the manuscript. Baljeet Kaur performed Transmission Electron Microscopy. All authors read and approved the final manuscript.
Ethics approval : This work does not contain any research involving humans or animals
Consent to participate : Not Applicable
Consent for publication : All the authors have approved the version to be published and given their consent for the publication of this paper.
Figure 1: Colony morphology (a) and growth pattern (b) of NR5 under different arsenic concentration
Figure 2: Langmuir (a) and Freundlich (b) isotherm for arsenic biosorption by NR5
Figure 3: Neighbour-joining tree based on 16S rRNA sequence of arsenic tolerant bacteria NR5 strain by MEGA 11. The scale bar corresponds to 0.02 substitutions per position. The numbers at nodes indicated the levels of bootstrap support (%) based on 1,000 resample data sets; only values above 50% are given.
Figure 4: Scanning Electron Microscopic image of B. mycoides NR5 without As (V) (control) (a); with 25 mg L-1 arsenic (c); EDS analysis of the isolate NR5, showing no arsenic in control (b); and significant arsenic distribution in the treated bacterial strain (d).
Figure 5: Transmission electron micrograph of (A) Bacillus mycoides NR5 grown in (a) nutrient broth (control) (magnification, ×20,000) and (b) in media containing 25 mg L-1 As (V) concentration (magnification, ×20,000).
Figure 6: Infrared spectra of (A) Bacillus mycoides NR5 grown in nutrient broth (control) and (B) media amended with 25 mg L–1 As (V) concentration.
Figure 7: Growth of spinach in the soil treated with As (V) and amended with the Bacillus mycoides NR5 at 60 DAI (a) Control: plants grown in the soil without any treatment; (b) plants grown in soil containing 25 mg kg–1 As (V) (c) plants grown in the soil containing 25 mg kg–1 As (V) inoculated withBacillus mycoides NR5