Methods
Saliva samples collected from 143 children (6-17 yrs) with
moderate-to-severe asthma across four European countries (the
Netherlands, Germany, Spain, and Slovenia) as part of the SysPharmPediA
cohort were subjected to 16S rRNA sequencing. Microbiome was compared
using global diversity (α and β) between two groups of participants
based on inhaler devices (MDI (n=77) and DPI (n=65)) and differential
abundance was compared using the Analysis of Compositions of Microbiomes
with the Bias Correction (ANCOM-BC) method.