B. B(a)P metabolism in CEGA

The viability in the group dosed with B(a)P at a total dose of 250 µg/egg was 100%. The viability in the vehicle control group was 100%.
The results of metabolism study following B(a)P exposure are shown in Figure 3. No relevant peaks were observed in either naïve (Figure 3A) or solvent control (Figure 3B) groups, indicating that no metabolites were formed in these groups. In contrast, in the livers of chicken embryo-fetuses that received injections with B(a)P, several peaks represent metabolites with transformations on B(a)P ring which were formed at sufficient levels (Figure 3C). The quantification of each transformation and type of transformation is listed in Table 1. These transformations when compared to the established metabolism of B(a)P in other species (Figure 4). Based on the suggested metabolite structures there seems to be similarity in the metabolites and amounts formed between chicken and rodent livers.