B. B(a)P metabolism in
CEGA
The viability in the group dosed with B(a)P at a total dose of 250
µg/egg was 100%. The viability in the vehicle control group was 100%.
The results of metabolism study following B(a)P exposure are shown in
Figure 3. No relevant peaks were observed in either naïve (Figure 3A) or
solvent control (Figure 3B) groups, indicating that no metabolites were
formed in these groups. In contrast, in the livers of chicken
embryo-fetuses that received injections with B(a)P, several peaks
represent metabolites with transformations on B(a)P ring which were
formed at sufficient levels (Figure 3C). The quantification of each
transformation and type of transformation is listed in Table 1. These
transformations when compared to the established metabolism of B(a)P in
other species (Figure 4). Based on the suggested metabolite structures
there seems to be similarity in the metabolites and amounts formed
between chicken and rodent livers.