Targeting the ADE2 gene in different species
The systematic names of orthologs of the ADE2 gene,YOR128C in S. cerevisiae, are shown in table 3 and were extracted from: http://www-archbac.u-psud.fr/genomes/nakaseomycetes/nakaseomycetes.html. Cut sites were chosen at a similar location in the gene (see table 3). Individual gRNAs were cloned into pCFYF for targeting the ADE2 gene in each species.