Time-course of CRISPR-Cas9 expression in liquid medium followed by cell rescue
Flasks containing 10mL of induction medium are inoculated with transformants grown overnight in liquid SC-Rep medium (as above) at OD = 0.1. Growth is followed by measuring optical density at 600nm. Rescue is performed at different time points by plating an aliquot on repressive medium. All experiments were performed on at least two independent transformants and were normalized with reference to transformants without the gRNA (transformation with pCFYF).