Sequence of cut-sites repaired by unfaithful NHEJ
We amplified the cut region by PCR in 19-20 red colonies per species, from the continuous induction experiments. As seen on figure 4, the large majority, 15/20, of unfaithful NHEJ events in C. glabrata create frameshift mutations by deletion of a single base pair. The remaining five cases consist of a frameshift mutation by deletion of 4 base pairs (one occurrence), addition of one base pair (three occurrences) and addition of two base pairs (one occurrence).
In the other two species, both insertions and deletions are found, that either create frameshifts or delete and/or modify a few codons at the cut site. The most frequent repaired sequence is found 6 times in each species, and corresponds to a single bp deletion in C. nivariensis , and to a single bp insertion in C. bracarensis .