PCR, DNA preparation, cloning
For analysis, PCR was performed directly on colonies, as described forC. glabrata in Boisnard et al. (2015), using the DreamTaq from ThermoFisher Scientific. For constructions, PCR was performed on DNA prepared with Qiagen Genomic DNA kits, according to manufacturer’s conditions. Some constructs were cloned into pBlueScript into E. coli DH5, with standard protocols.