Time-course of CRISPR-Cas9 expression in liquid medium followed by
cell rescue
Flasks containing 10mL of induction medium are inoculated with
transformants grown overnight in liquid SC-Rep medium (as above) at OD =
0.1. Growth is followed by measuring optical density at 600nm. Rescue is
performed at different time points by plating an aliquot on repressive
medium. All experiments were performed on at least two independent
transformants and were normalized with reference to transformants
without the gRNA (transformation with pCFYF).