Exosome loading with quercetin
Approximately, 99ml of exosomes extracted from the mare’s milk mixed
with 14ml of quercetin dissolved in 1% DMSO solution to a achieve a
final concentration of 160μM. The solution was left to mix with magnet
stirrer for 24 hours. In order to get rid of unincorporated quercetin
and purify the solution, IZON ASF and qEV original 70 (IZON Science, New
Zealand) columns were used.