Cell cultures
Neonatal human dermal fibroblasts (Gibco) cells were cultured in DMEM
medium high glucose with L-glutamine and with sodium pyruvate (Biowest,
France), supplemented with 10% fetal bovine serum exosome-depleted
(Gibco; Thermo Fisher Scientific, Inc.) and 1% Penicillin-Streptomycin
solution (Sigma) in a 37°C, 5% CO2 incubator (Binder
CB-150, Binder, Germany). To induce aging, cells with
3μM of doxorubicin (DOX) for 3 hours, and then washed with HBSS. To
determine the effect of exosomes, cells were incubated with exosomes
alone from different isolation method (IP, SEC, or TEI) and loaded with
quercetin and compared to quercetin only using different concentrations
20, 40, 80, and 160μM. For vehicle control cell were treated with 1%
DMSO, and for negative control, cells were treated with 1 × PBS.