Exosome loading with quercetin
Approximately, 99ml of exosomes extracted from the mare’s milk mixed with 14ml of quercetin dissolved in 1% DMSO solution to a achieve a final concentration of 160μM. The solution was left to mix with magnet stirrer for 24 hours. In order to get rid of unincorporated quercetin and purify the solution, IZON ASF and qEV original 70 (IZON Science, New Zealand) columns were used.