Transmission electron microscopy
Approximately, five µL of isolated exosomes using SEC, IP and TEI added onto a Carbon Film Supported Copper Grid (Sigma-Aldrich) for Transmission Electron Microscopy (TEM) analysis. The concentrated samples, maintained in a sterile environment, and kept still in order to allow the liquid to evaporate. Samples then were fixated and characterized using JEM-1400 Plus electron microscope.