Transmission electron microscopy
Approximately, five µL of isolated exosomes using SEC, IP and TEI added
onto a Carbon Film Supported Copper Grid (Sigma-Aldrich) for
Transmission Electron Microscopy (TEM) analysis. The concentrated
samples, maintained in a sterile environment, and kept still in order to
allow the liquid to evaporate. Samples then were fixated and
characterized using JEM-1400 Plus electron microscope.