Mare’s Milk purchase
Fresh mare’s milk purchased from Saumal factory located at Karaganda region, Kazakhstan, and aliquoted in 50 ml tubes then stored at -80 ºC. To remove milk fat globules and cell debris, thawed samples were centrifuged at 10000 × g for 30 minutes at 4 ºC. To obtain large number of good quality exosomes, three exosome isolation methods were used.