Sections stained with Periodic Acid-Schiff (PAS) revealed a PAS-positive reaction (staining purple-red) in the basal membranes and areas of thickened Bowman’s capsule in all animals, an increase in the number of PAS-stained glomeruli with thickened mesangial matrix and basal membranes, glomerulosclerosis, tubular casts, and PAS-positive thickened tubular basal membranes (Figure 8).