Figure 3 Evolution of sws1 opsin genes in damselfishes, Pomacentridae. A) Bayesian phylogenetic tree of the relationship betweensws1 copies of eleven damselfish species. Annotated sws1 copies of the false percula clownfish Amphiprion ocellaris (*) were used to infer the identity of the damselfish genes (Mitchell et al., 2021a). The amino acid (AA) identities at two key SWS1 spectral tuning sites, 114 and 118 (bovine rhodopsin numbering; Shi and Yokoyama, 2003; Yokoyama, 2000), are shown on the right. Each SWS1 opsin was classified as short- or long-wavelength sensitive based on the predictions of Stieb et al. 2024. In grey are SWS1 outgroup orthologs used for phylogenetic reconstruction showing the peak-spectral sensitivities (λmax) they convey. Note that, gene conversion between the Neoglyphidodon melas sws1 copies has pulled sws1β towards the sws1a cluster and has led to an AA switch at the two tuning sites (also see Figs. 6 and S2 for details).