Figure Legends
Figure 1 . Sampling of Phyllotis vaccarum across an
elevational gradient in the Altiplano and Puna de Atacama of northern
Chile, Regiόn de Antofagasta. (a) Map of seven collection localities on
the flanks of Volcán Lullaillaco and the surrounding Altiplano. (b)
Elevational profile of sampling transect, with sampling localities 1 to
7 shown in ascending order of elevation, from 2370 m (site 1) to 6739 m
(the summit of Volcán Lullaillaco, site 7). (c) Northwest face of Volcán
Llullaillaco (24°43.21′S, 68°32.22′W). Photo was taken from a point
several km northwest of the site 3 on the transect. Photo: J.F. Storz.
Figure 2 . Schematic figure of the dissected portions of the
gastrointestinal tract of the mouse from the summit of Volcán
Llullaillaco. Contents of the upper digestive tract (stomach) were
analyzed via metagenomics and DNA metabarcoding. Contents of the lower
digestive tract (separated into the cecum and 12 consecutive segments
from the outlet of the cecum to the rectum) were analyzed via DNA
metabarcoding.
Figure 3 . Taxonomic composition of reads identified through
metabarcoding for the stomach, cecum and 12 consecutive portions of the
lower GI from the outlet of the cecum to the rectum. (A) Proportion of
sequence reads per taxon for the marker trn and (B) proportion of
total reads for the same marker with Erythroxylaceae excluded. (C)
Proportion of sequence reads per taxon for the marker ITS2 and
(D) proportion of total reads for the same marker with Erythroxylaceae
excluded. (E) Proportion of sequence reads per taxon for the marker23S and (F) proportion of total reads for the same marker with
Erythroxylaceae excluded.
Figure 4 . Taxonomic composition of Ascomycota (A) and
Basidiomycota (B) at the Order and Family levels.
Figure 5. Variation in stable isotopes in livers of Phyllotis
vaccarum sampled from different elevational zones: (A)
δ13C, (B) δ15N, and (C)
δ34S. (D) Results of multivariate CAP ordination based
on Euclidean distances calculated from combined δ13C,
δ15N and δ34S values.
Figure 6. Variation in plant δ13C (A) and
δ15N (B) across different elevational zones. Values
taken from Díaz et al. (2016). Note the elevational shift in
δ13C values showing dominance of C4plants at lower elevations, a mix of C3, C4 and CAM plants at
mid-elevations and a shift to C3 plants at higher elevations. Plant
δ15N values were similar at lower and mid-elevations
but were relatively 15N depleted at higher elevations.
These data were used to define isotopic baselines for estimates of
trophic position of P . vaccarum .