Graphical Abstract
Line-field Confocal Optical Coherence Tomography (LC-OCT) is used to determine the human skin in vivo optical properties modifications caused by biocompatible optical clearing. After a prior calibration using a phantom with determined optical properties, a theoretical model is applied to mean in-depth intensity profiles of 3D LC-OCT images acquired during optical clearing protocol. As a result, kinetic relative changes of epidermal and dermal layer optical properties demonstrated with the best results caused by the mixture of Polyethylene Glycol, Oleic Acid and Propylene Glycol.