Effect of temperature and salinity on maximum germination

Mean germination was affected by both location and temperature treatment, however, there was no interaction between the two covariates, highlighting that thermally affected seeds did not respond differently to temperature treatments. Salinity increased germination (p = 0.035, Table 2) but did not have any significant interaction effects between treatment and source. Emmeans post hoc highlighted that all treatments had significantly less germination when compared to the 16°C treatment but were not different from each other (Figure 4; Table 2). Further, there were significant differences between the 16°C treatments held at 32 ppt salinity in thermally affected locations compared to the 16°C in ambient locations, with germination rates of 5.5% (± 1.08) and 2.8 (±.58) respectively (Figure 4).