Reduced activity in influenza vaccinated mice can be prevented by vaccination. CB6F1 Mice (3-6 per group) were vaccinated with a single dose of hemagglutinin (PR8) formulated in CAF01. 21 days later, the mice were challenged with 150 EID50 of A/PR/8 virus. 6 days before challenge, the mice were tagged with RFID transponders. The average activity during the 6-day time period was used to set baseline activity for individual mice. Following challenge, the mice were followed for 7 days before termination. Weight loss and scoring was measured daily and activity was continuously monitored. Determination of viral titers in nasal washes (days 4 and 7) and lungs (day 7) was measured by RT-PCR. Error bars show standard deviation. Statistical analysis was performed by two-tailed unpaired t-test comparing unvaccinated and HA+CAF01 immunized mice for every time point (A-C). Correlation was analyzed by Pearson correlation (D) A two-tailed unpaired t-test was used to compare unvaccinated and HA+CAF01 immunized mice (E). * p-value ≤ 0.05, ** p-value ≤ 0.01, *** p-value ≤ 0.001, **** p-value ≤ 0.0001.