Mice
CB6F1 mice were ordered from Envigo (Horst, The Netherlands). Six–eight
weeks-old female mice were used in all experiments. All mice were housed
in the animal facilities at Statens Serum Institut, Denmark, and
maintained in rooms with a controlled environment (20–23 ℃; relative
humidity 55 ± 10 %; 12/12 h light/dark cycle). The mice were randomly
allocated to conventional type III polycarbonate cages (820
cm2). All animals were provided Aspen bedding and
bricks (Tapvei), EnviroDri (LBS), and polycarbonate tunnels or houses or
DesRes paper houses (LBS). Irradiated sunflower seeds, corn grains, and
peanuts or bits of carrots were given to the animals once a week. A
pelleted diet (Envigo Teclad 2916) and tap water were provided ad
libitum. Mouse studies were conducted following the regulations set
forth by the National Animal Protection Committee and following European
Community Directive 86/609. The governmental Animal Experiments
Inspectorate has approved the experiments performed under licenses
2020-15-0201-00525.