2.3.1 RNA-Seq-based transcriptome data
On the 60th day of the experiment, eight to ten mature healthy leaves ofS. krylovii per population per soil moisture treatment were
collected and sent to Shanghai MajorBio Technology Co., Ltd for
RNA-Seq-based transcriptome analysis after distilled water washing and
liquid nitrogen freezing. Total RNA was extracted from the tissue using
TRIzol® Reagent and quantified using the ND-2000 (NanoDrop
Technologies), and then was sequenced with the NovaSeq 6000 sequencer (2
× 150bp read length).