2.3.1 RNA-Seq-based transcriptome data
On the 60th day of the experiment, eight to ten mature healthy leaves ofS. krylovii per population per soil moisture treatment were collected and sent to Shanghai MajorBio Technology Co., Ltd for RNA-Seq-based transcriptome analysis after distilled water washing and liquid nitrogen freezing. Total RNA was extracted from the tissue using TRIzol® Reagent and quantified using the ND-2000 (NanoDrop Technologies), and then was sequenced with the NovaSeq 6000 sequencer (2 × 150bp read length).