Molecular cloning and virus packing
The CMV promoter drives the expression of TRPV1 and mCherry, which was
separated by a 2A sequence followed by a stop sequence. TRPV1 DNA
fragments inserted with a 6 × His tag were synthesized and cloned into
plasmids. The plasmid was packed into the Lenti virus according to
established protocols. Before use, all the viral vectors were diluted to
a titer of 1012 transducing units per milliliter and
used for neuronal cell transduction.