Fig. 4 Virus and FVIOs injection into the mouse CeA for the
magnetothermal neuromodulation of fear behaviors. (a) Scheme of
anti-His-FVIO-based magnetothermal neurostimulation of the CeA and fear
behavior analysis of freely moving mice. (b) Confocal images of brain
slices showing that the anti-His-FVIOs-FL (green) are located in the
TRPV1-expressing CeA regions (red) of the mice. (c, d) Percentage of
freezing time in mice after injection with different doses of
anti-His-FVIOs (or anti-His-SPIOs) before and during AMF stimulation (H
= 20 mT, f = 275 kHz). The black and red (or green) lines
represent AMF OFF and AMF ON, respectively. One-way ANOVA was performed
followed by a two-sided Student’s test with thresholds. (e) Latency to
the start of the first freezing episode during AMF stimulation in mice.
(f) DAPI (blue), c-fos (green) and overlay confocal images of the CeA
regions of mice obtained after magnetothermal stimulation. (g)
Percentage of c-fos-expressing cells in the CeA among the DAPI-labeled
cells. Error bars indicate the mean ± SEM, two-sided Student’s t-test
with the threshold ****P < 0.0001.