Fig.3-3 The expression levels of MareA gene in C100,
ΔMareA and OE-MareA strains
3.2 Phenotypic analysis of ΔMareA and OE-MareA strains
3.2.1 Morphological analysis of colonies
Based on the combined data presented above, it is evident that the
knockout strains ΔMareA exhibit a more pronounced difference in
colony morphology compared to the original strain C100. Regarding aerial
mycelium production, the overexpression strain OE-MareA demonstrates increased levels, while the ΔMareA strain displays
significantly reduced aerial mycelium formation. There is minimal
variation in colony color among the three strains. When comparing
different nitrogen sources, Gln and
(NH4)2SO4 have
negligible impact on colony morphology, whereas NaNO3and Urea exert a more significant effect. Specifically, growth of the
ΔMareA strain is markedly inhibited on PDA plates supplemented
with NaNO3 and Urea, while colonies of OE-MareA strain exhibit normal growth. In conclusion, it can be inferred that
knockdown or overexpression of MareA gene influences colony
morphology without substantial impact on pigmentation species.
Additionally, different nitrogen sources exert varying degrees of
influence on colony morphology for all three strains. As shown in Figure
3-4.