Fig.3-6 Conidial numbers of C100, ΔMareA and
OE-MareA in medium with different nitrogen sources
3.2.4 Microscopic Morphological Analysis
In PDA medium, C100 mycelium had septa and a few nuclei. ΔMareA mycelium was slender, with a long distance between septa and few nuclei.
OE-MareA mycelium was thicker and had more nuclei, and
OE-MareA mycelium exhibited a stronger fluorescence signal. In
PDA-Gln medium, both ΔMareA and OE-MareA mycelium were
thicker compared to C100, and all three strains showed stronger
fluorescence signals. On
PDA-(NH4)2SO4 medium,
the three strains did not differ significantly, but the fluorescence
intensity of C100 was slightly stronger than that of ΔMareA and
OE-MareA strains. In PDA-NaNO3 medium, the
mycelium of C100 and OE-MareA was thicker than that of
ΔMareA . The fluorescence intensity of C100 was the strongest,
followed by OE-MareA , while ΔMareA had the weakest
intensity. The fluorescence intensity of C100 and OE- MareA in
PDA-Urea medium was significantly stronger than that of ΔMareA strain. Overall,the three strains exhibited different morphologies
under different culture conditions along with varying levels of
fluorescence intensities,with the most significant differences observed
in the strain supplemented with NaNO3.This suggests that
the MareA gene can influence monascus’ growth and
development,and at the same time,the MareA gene utilizes
different nitrogen sources differently which subsequently affects
monascus’ growth. As shown in Figure 3-7.