Figure legends
Figure 1 . Three- and seven-days growth of a marine microbial community on LDPE, and analysis of biofilm structure and functioning. ASW+G, artificial seawater supplemented with vitamins, trace metals, and glucose; BHB+v, Bushnell Haas Broth supplemented with vitamins and trace metals.
Figure 2. Relative abundance of microbial species found within the metaproteome at each time point. Calculated using the number of proteins associated with each genus relative to all the proteins found in that condition. Values used for doughnut plot shown in table below, as well as corresponding metagenomic data.
Figure 3. Total differentially regulated proteins (FC>1.5, P-adj. <0.05) according to function and taxonomic classification. Positive fold change (purple) in a indicates greater abundance of proteins on D7 (purple), and negative fold change (orange) indicates greater expression on D3.
Figure 4. Graphic representation of biologically relevant processes found within this study’s most abundant bacterial genera from relative quantification and qualitative analysis of the metaproteomic dataset. Purple = more expressed on day 7, orange = more expressed on day 3, black = qualitative results, * = qualitative results with 1 entry, grey = not found, but can be assumed. Colour code indicates all basal processes active within the genus at that time (>1 protein each). All basal processes active in the largest, annotated microorganisms. Acronyms: Figure elements : H2O2, hydrogen peroxide; HGT, horizontal gene transfer; NO, nitric oxide; O2, superoxide; PE, polyethylene; PHA, polyhydroxyalkanoate granule; Xn, xenobiotic. Stress (osmotic, oxidative, heat/cold, interspecies interaction, xenobiotic): Ahp, alkyl hydroperoxide reductase; ArnA, polymyxin resistance protein; α2M, alpha-2-macroglobulin; β-L, β-lacatamse; Cap, capsular biosynthesis protein; CAT, catalase; CopA, copper resistance protein A; CSD, cold shock domain containing protein; Fhp, flavohemoprotein; FtsH, ATP-dependent zinc metalloprotease; G6PD, glucose-6-phosphate dehydrogenase; Gpx, glutathione peroxidase; GrpE, DnaK nucleotide exchange factor; GSH, glutathione; HU, transcriptional regulator HU; Kat, catalase peroxidase (also PE degradation) ; LonP, lon protease; MsrP, methionine sulfoxide reductase; OP, 5-oxoprolinase; OprM, outer membrane efflux system protein; Osm, osmotically inducible protein; Psp, phage shock protein; RdgC, recombination-associated protein; RecA, SOS response protein ; SOD, superoxide dismutase; OpuAC, substrate binding domain of ABC-type glycine betaine transport system; TerD, tellurium resistance protein; TP, thiol peroxidase; TPx, thioredoxin peroxidase; Trx, thioredoxin; TrxR, thioredoxin reductase; Chaperones- ClpA, ClpB, DnaJ, DnaK, HtpG, SurA; Chaperonins- GroEL, GroES. Virulence and biofilm formation : 1B, hemolysin co-regulated protein; BepC, outer membrane efflux protein; Ccda, control of cell death protein; ComEA, competence protein; EL-Tu, elongation factor Tu; hcp, hemolysin-coregulated protein; Hly, hemolysin activation/ secretion protein; Omp, outer membrane protein; RND, efflux resistance-nodulation-division (RND) transporter; SecYEG, translocon; TISS, type-I secretion system; TIISS, type-II secretion system; TIVSS, type-IV secretion system; TVISS, type-VI secretion system; VgrG2b, actin cross-linking spike protein; Translocons- IcmF, Tld/PmbA, Vask, VipB.Starvation : CobW, cobalamin biosynthesis protein; PhaF, polyhydroxyalkanoate (Pha) granule-associated protein F; Phal, Pha granule-associated protein L; PHAs, PHA synthase; SSP, stringent starvation protein. Adhesion : AidA, adhesin; CGC, calcium-gated channel; CsgG, curli assembly protein G; LAD, large adhesive protein; LP, lipoprotein; OAA, OAA-family lectin sugar binding domain containing protein; TrkA, Potassium uptake protein. Aromatic hydrocarbon degradation : 3-OCoAT, 3-oxoadipate-CoA transferase; 3-HA-CoAd, 3-hydroxyacyl-CoA dehydrogenase; ACC, acetyl-CoA carboxylase; A-CoAAT, acetyl-CoA acetyltransferase; A-CoAD, acetyl-CoA dehydrogenase; CMBL, dienelactone hydrolase; PA, pyruvate adolase; PCMH-p, p-crestol methylhydroxylase (PCMH) type protein; TI, thiolase I; TMPT, thiopurine s-methyltransferase. PE degradation : AD, alcohol dehydrogenase; ALDH, aldehyde dehydrogenase.