2.4 Phylogenetic tree construction
In order to explore the phylogenetic relationships of the genes encoding
12 structural enzymes in the GSH pathway of G. hirsutum , a
maximum likelihood (ML) tree was constructed based on the amino acid
sequences of these genes under the Jones-Taylor-Thornton (JTT) model
with 500 bootstrap replications using the MEGA (v. 11) software. FigTree
v1.4.4 (http://tree.bio.ed.ac.uk/software/figtree/) and Adobe
Illustrator 2022 was used to visualize and edit the ML tree,
respectively.