3.6 qRT-PCR verification
To confirm the reliability and validity of the results of
RNA-sequencing, nine structural genes and their potential eight
regulatory genes (Table S2) were randomly selected for qRT-PCR analysis
under CK and UV-B stress conditions. As expected, the qRT-PCR profiles
showed consistent results with RNA-sequencing, with 15 up-regulated and
only two (GGT3 and IDH ) down-regulated expression under
UV-B stress (Table S5, S7). In general, the expression of regulatory
genes was more enhanced than that of structural genes. However, the
expression levels of a structural genes, GSTU8 , were higher than
their regulatory genes after UV-B stress (Fig. 4).