2.5 RNA extraction and transcriptome sequencing
Total RNAs of leaves in the CK and UV-B stress treatment (UV) groups
were extracted using RNAprep Pure Plant Kits (Cat. No 4992237, TIANGEN,
Beijing, China) following the manufacturer’s instructions, respectively.
RNA integrities and quantities were assessed using the RNA Nano 6000
Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, CA,
USA).
Six cDNA sequencing libraries (each three replicates of CK and UV) were
constructed using NEB Next® Ultra™ RNA Library Prep Kit for Illumina®
(New England Biolabs, MA, USA) following the manufacturer’s
recommendations. The library fragments were purified using the AMPure XP
system (Beckman Coulter, Beverly, USA) and sequenced on an Illumina
NovaSeq 6000 platform. Library construction and transcriptome sequencing
were achieved by Beijing Novogene Bioinformatics Technology Co. Ltd.