Figure legends
Fig. 1. Stress treatment, histochemical staining and physiological index analysis of G. hirsutum . A-C, The NBT assay, Evans Blue stain, and DAB stain were used to visualize the content of O2•- in leaves, cell damage and the content of H2O2 in leaves. D-E, The reaction of hydroxylamine and superoxide anion radicals and hydrogen peroxide kit were used to detect the content of O2•- and H2O2 in leaves between CK and UV-B group. F-G, Plant phenotypes under natural condition for 6 h (CK) or UV-B (16 kJ m-2 d-1) (UV-B) for 6 h.
Fig. 2. A ML tree of 12 structural gene families of G. hirsutum. The tree was constructed based on the amino acid sequences of 205 structural genes with 500 bootstrap replicates. Different family or subfamily clades are shadowed by different colors.
Fig. 3. Pathway diagram and expression heat map of structural and regulatory genes related to the GSH metabolism. Pathway diagram shows 12 key enzymes in the GSH metabolic pathway; heat map shows structural genes with significantly differential expression under UV-B stress.
Fig. 4. qRT-PCR analysis of expression changes of representative structural genes and their regulatory genes in the GSH metabolic pathway under UV-B stress. A-F, The relative expression of G6PDH , GGT , GPX , IDH ,GST , and PGDC , and their regulatory genes. Statistical significance was calculated using T -test; * P < 0.05, ** P < 0.01.