2.8 Functional enrichment of DEGs
Gene Ontology (GO) term clustering and Kyoto Encyclopedia of genes and
genomes (KEGG) pathways enrichment analysis of the DEGs was implemented
by the clusterProfiler R package (Yu et al., 2012), in which gene length
bias was corrected. The GO terms and KEGG pathway with correctedPadj < 0.05 were considered significantly enriched in
the DEGs. Based on the results of GO and KEGG enrichment analysis, the
genes that were differentially expressed in the GSH metabolic pathway
under UV-B treatment were identified. The online website ImageGP
(https://www.bic.ac.cn/ImageGP/) was used to draw a heatmap of
DEGs using normalized expression values of log2(FPKM+1).
2.9 Expression level validation of the DEGs by