Figure legends
Fig. 1. Stress treatment, histochemical staining and
physiological index analysis of G. hirsutum . A-C, The
NBT assay, Evans Blue stain, and DAB stain were used to visualize the
content of O2•- in leaves, cell
damage and the content of H2O2 in
leaves. D-E, The reaction of hydroxylamine and superoxide anion
radicals and hydrogen peroxide kit were used to detect the content of
O2•- and
H2O2 in leaves between CK and UV-B
group. F-G, Plant phenotypes under natural condition for 6 h
(CK) or UV-B (16 kJ m-2 d-1) (UV-B)
for 6 h.
Fig. 2. A ML tree of 12 structural gene families of G.
hirsutum. The tree was constructed based on the amino acid sequences of
205 structural genes with 500 bootstrap replicates. Different family or
subfamily clades are shadowed by different colors.
Fig. 3. Pathway diagram and expression heat map of structural
and regulatory genes related to the GSH metabolism. Pathway diagram
shows 12 key enzymes in the GSH metabolic pathway; heat map shows
structural genes with significantly differential expression under UV-B
stress.
Fig. 4. qRT-PCR analysis of expression changes of
representative structural genes and their regulatory genes in the GSH
metabolic pathway under UV-B stress. A-F, The relative
expression of G6PDH , GGT , GPX , IDH ,GST , and PGDC , and their regulatory genes. Statistical
significance was calculated using T -test; * P <
0.05, ** P < 0.01.