2.4 Phylogenetic tree construction
In order to explore the phylogenetic relationships of the genes encoding 12 structural enzymes in the GSH pathway of G. hirsutum , a maximum likelihood (ML) tree was constructed based on the amino acid sequences of these genes under the Jones-Taylor-Thornton (JTT) model with 500 bootstrap replications using the MEGA (v. 11) software. FigTree v1.4.4 (http://tree.bio.ed.ac.uk/software/figtree/) and Adobe Illustrator 2022 was used to visualize and edit the ML tree, respectively.