2.3 | Laboratory Testing
Pre-transplant multiplex RV PCR testing was performed twice–once up to
10 days leading up to admission and once upon admission. Multiplex RV
PCR is the current clinical recommendation to detect symptomatic
respiratory viruses pre-HSCT.24 Samples were
considered positive if one or more virus was detected. At
Children\RL’s National Hospital, this RV PCR is a BioFire FilmArray
(bioMérieux) which includes adenovirus, coronavirus, SARS CoV19,
human metapneumovirus (HMPV), rhinovirus/enterovirus, influenzae A and
B, parainfluenzae 1-4, and RSV A and B. These tests were performed
within a week of and on the day of admission for the HSCT procedure. The
result of the RV PCR and the presentation of symptoms were factored into
the decision to proceed with transplantation.