3. Results
To gain insight into CB1Rs functions specifically in inhibitory neural
circuits of the central auditory system, we selectively deletedCnr1 by Cre dependent deletion of loxP flanked exons, which leads
to an unstable protein (Marsicano et al., 2002, 2003).Cnr1flox/flox mice were mated with PV-Cre mice
to generate mice with CB1Rs cKO specifically from PV interneurons.Cnr1flox/flox mice were used as controls
because they retain normal levels of CB1Rs and functionality
(Fig. S1c ). Mice with two different genotypes,
Cnr1flox/flox (non-cKO) and
PV-Cre;Cnr1flox/flox (Cnr1-cKO), were
identified and randomly separated into several groups for different
purposes.