To obtain a more quantitative and detailed understanding of CB1Rs distribution, the average fluorescence intensities of CB1Rs across distinct auditory nuclei were analyzing for quantifying CB1Rs expression patterns (Fig. 1c, 2b). Average fluorescence intensities of CB1Rs are maximumly distributed in the regions of the SOC and CN, compared to the cell number calculations (Fig. 1c). While the cell numbers in the LL were almost the same as in the SOC, the average fluorescence intensity of CB1Rs in the LL was closer to the CN than to the SOC (Fig. 1c). LSO had a greater number of CB1R+ neurons compared to MNTB and MSO (medial superior olive), yet the average fluorescence intensities of CB1Rs were similar across the three nuclei (Fig. 2b, 2c). The inconsistency between cell number and fluorescence intensity observed in the subregions of CN and IC further highlights the complexity of CB1Rs expression patterns across different auditory nuclei (Fig. 2b, 2c). The discrepancy between the analysis of cell number and fluorescence intensity of CB1Rs expression can indeed be attributed to a variety of factors, including the differential enrichment of CB1Rs expression in distinct brain regions and cell types, as well as the localized distribution of CB1R+ neurons within specific regions.