4. Discussion
In the current study, we assessed region-specific distribution of the CB1Rs across the central auditory system of mice using fluorescence immunohistochemical techniques. Our results revealed that CB1Rs are highly expressed in the CN, SOC and LL. Furthermore, the conditional knockout of CB1Rs specifically from PV interneurons resulted in hearing loss and abnormal auditory brainstem response to a certain extent. Our findings support a functional role for CB1Rs in regulating inhibitory circuitry function in the central auditory system, ultimately impacting auditory processing.