3. Results
To gain insight into CB1Rs functions specifically in inhibitory neural circuits of the central auditory system, we selectively deletedCnr1 by Cre dependent deletion of loxP flanked exons, which leads to an unstable protein (Marsicano et al., 2002, 2003).Cnr1flox/flox mice were mated with PV-Cre mice to generate mice with CB1Rs cKO specifically from PV interneurons.Cnr1flox/flox mice were used as controls because they retain normal levels of CB1Rs and functionality (Fig. S1c ). Mice with two different genotypes, Cnr1flox/flox (non-cKO) and PV-Cre;Cnr1flox/flox (Cnr1-cKO), were identified and randomly separated into several groups for different purposes.